Blue native PAGE: Applications to study peroxisome biogenesis

研究成果: 著書/レポートタイプへの貢献

抄録

Blue native polyacrylamide gel electrophoresis (BN-PAGE) is one of the useful methods to isolate protein complexes including membrane proteins under native conditions. In BN-PAGE, Coomassie Brilliant Blue G-250 binds to proteins and provides a negative charge for the electrophoretic separation without denaturing at neutral pH, allowing the analysis of molecular mass, oligomeric state, and composition of native protein complexes. BN-PAGE is widely applied to the characterization of soluble protein complexes as well as isolation of membrane protein complexes from biological membranes such as the complexes I–V of the mitochondrial respiratory chain and subcomplexes of the mitochondrial protein import machinery. BN-PAGE has also been introduced in the field of peroxisome research, for example, analysis of translocation machinery for peroxisomal matrix proteins embedded in the peroxisomal membrane. Here, we describe a basic protocol of BN-PAGE and its application to the study of peroxisome biogenesis.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ197-205
ページ数9
DOI
出版物ステータス出版済み - 1 1 2017

出版物シリーズ

名前Methods in Molecular Biology
1595
ISSN(印刷物)1064-3745

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Native Polyacrylamide Gel Electrophoresis
Peroxisomes
Proteins
Membrane Proteins
Membranes
Mitochondrial Proteins
Electron Transport
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Okumoto, K., Tamura, S., & Fujiki, Y. (2017). Blue native PAGE: Applications to study peroxisome biogenesis. : Methods in Molecular Biology (pp. 197-205). (Methods in Molecular Biology; 巻数 1595). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6937-1_18

Blue native PAGE : Applications to study peroxisome biogenesis. / Okumoto, Kanji; Tamura, Shigehiko; Fujiki, Yukio.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 197-205 (Methods in Molecular Biology; 巻 1595).

研究成果: 著書/レポートタイプへの貢献

Okumoto, K, Tamura, S & Fujiki, Y 2017, Blue native PAGE: Applications to study peroxisome biogenesis. : Methods in Molecular Biology. Methods in Molecular Biology, 巻. 1595, Humana Press Inc., pp. 197-205. https://doi.org/10.1007/978-1-4939-6937-1_18
Okumoto K, Tamura S, Fujiki Y. Blue native PAGE: Applications to study peroxisome biogenesis. : Methods in Molecular Biology. Humana Press Inc. 2017. p. 197-205. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6937-1_18
Okumoto, Kanji ; Tamura, Shigehiko ; Fujiki, Yukio. / Blue native PAGE : Applications to study peroxisome biogenesis. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 197-205 (Methods in Molecular Biology).
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