Blue native PAGE: Applications to study peroxisome biogenesis

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

Blue native polyacrylamide gel electrophoresis (BN-PAGE) is one of the useful methods to isolate protein complexes including membrane proteins under native conditions. In BN-PAGE, Coomassie Brilliant Blue G-250 binds to proteins and provides a negative charge for the electrophoretic separation without denaturing at neutral pH, allowing the analysis of molecular mass, oligomeric state, and composition of native protein complexes. BN-PAGE is widely applied to the characterization of soluble protein complexes as well as isolation of membrane protein complexes from biological membranes such as the complexes I–V of the mitochondrial respiratory chain and subcomplexes of the mitochondrial protein import machinery. BN-PAGE has also been introduced in the field of peroxisome research, for example, analysis of translocation machinery for peroxisomal matrix proteins embedded in the peroxisomal membrane. Here, we describe a basic protocol of BN-PAGE and its application to the study of peroxisome biogenesis.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ197-205
ページ数9
DOI
出版ステータス出版済み - 1 1 2017

出版物シリーズ

名前Methods in Molecular Biology
1595
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

フィンガープリント 「Blue native PAGE: Applications to study peroxisome biogenesis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル