Bone-like nodules formed in vitro by rat periodontal ligament cells

M. Mukai, Y. Yoshimine, A. Akamine, K. Maeda

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

The periodontal ligament has been shown to possess the ability to regenerate both new cementum and alveolar bone as well as a self-regenerative capacity; however, the source of cementoblasts and osteoblasts is not still clear. We investigated the development of bone-like tissue in vitro by periodontal ligament cells, in order to determine whether the periodontal ligament contains osteoprogenitor cells. Periodontal ligament cells were obtained from periodontal ligament tissue attached to the maxillary incisors of 6-week-old WKA rats by means of the explant technique. Cells at passage #3 were cultured for long term in α-minimum essential medium containing 10% fetal bovine serum, antibiotics, and 50 μg/ml ascorbic acid, and were then examined using phase-contrast microscopy, histochemistry, transmission electron microscopy, X-ray microanalysis, and electron diffraction. Nodules were formed in the cultures, and when 10 mM Na-β-glycerophosphate was added, these nodules became mineralized. The mineralized nodules were identified as bone-like elements in view of the presence of osteoblast-like and osteocyte-like cells, collagenous matrix, a mineral composed of hydroxyapatite, and intense alkaline phosphatase activity. The results show that the periodontal ligament contains osteoprogenitor cells, which differentiate into osteoblasts and produce bone-like tissue.

本文言語英語
ページ(範囲)453-460
ページ数8
ジャーナルCell & Tissue Research
271
3
DOI
出版ステータス出版済み - 3 1 1993

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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