Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force

Kazunori Shimizu, Akira Ito, Tatsuro Yoshida, Yoichi Yamada, Minoru Ueda, Hiroyuki Honda

研究成果: ジャーナルへの寄稿学術誌査読

132 被引用数 (Scopus)

抄録

An in vitro reconstruction of three-dimensional (3D) tissues without the use of scaffolds may be an alternative strategy for tissue engineering. We have developed a novel tissue engineering strategy, termed magnetic force-based tissue engineering (Mag-TE), in which magnetite cationic liposomes (MCLs) with a positive charge at the liposomal surface, and magnetic force were used to construct 3D tissue without scaffolds. In this study, human mesenchymal stem cells (MSCs) magnetically labeled with MCLs were seeded onto an ultra-low attachment culture surface, and a magnet (4000 G) was placed on the reverse side. The MSCs formed multilayered sheet-like structures after a 24-h culture period. MSCs in the sheets constructed by Mag-TE maintained an in vitro ability to differentiate into osteoblasts, adipocytes, or chondrocytes after a 21-day culture period using each induction medium. Using an electromagnet, MSC sheets constructed by Mag-TE were harvested and transplanted into the bone defect in the crania of nude rats. Histological observation revealed that new bone surrounded by osteoblast-like cells was formed in the defect area 14 days after transplantation with MSC sheets, whereas no bone formation was observed in control rats without the transplant. These results indicated that Mag-TE could be used for the transplantation of MSC sheets using magnetite nanoparticles and magnetic force, providing novel methodology for bone tissue engineering.

本文言語英語
ページ(範囲)471-480
ページ数10
ジャーナルJournal of Biomedical Materials Research - Part B Applied Biomaterials
82
2
DOI
出版ステータス出版済み - 7月 2007

!!!All Science Journal Classification (ASJC) codes

  • 生体材料
  • 生体医工学

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