抄録
Bongkrekic acid (BKA) is shown to inhibit adenine nucleotide translocator in the mitochondrial inner membrane, which is bifunctional and facilitates ATP/ADP exchange and permeability transition pore opening in mitochondria. We demonstrate that BKA reduces the viability of tumor cells depending on the cell number in the culture. This reduction was first demonstrated by Cell Counting Kit-8 (CCK-8), which indicates cellular viabilities based on the reduction of a formazan dye by cellular NADH. Reduced viability was not seen in normal cells. The glycolysis inhibitor 2-deoxyglucose enhanced the effect of BKA. BKA is thought to work as a mitochondrial inhibitor; however, its action is different from mitochondrial uncouplers. We further demonstrate that BKA suppresses autophagy induction, directing cells to death in nutrient-depleted conditions. Together these data indicate that BKA is a new type of mitochondrial inhibitor that does not disrupt the mitochondrial membrane and that is thus used together with a glycolysis inhibitor as an anticancer agent.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 23-27 |
| ページ数 | 5 |
| ジャーナル | Evergreen |
| 巻 | 4 |
| 発行部数 | 2-3 |
| DOI | |
| 出版物ステータス | 出版済み - 9 2017 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Surfaces, Coatings and Films
- Management, Monitoring, Policy and Law
これを引用
Bongkrekic acid induces selective cytotoxicity in tumor cells, revealed by CCK-8. / Kano, Arihiro; Kamita, Moses Kibunja; Iwasaki, Takuma; Shindo, Mitsuru.
：: Evergreen, 巻 4, 番号 2-3, 09.2017, p. 23-27.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Bongkrekic acid induces selective cytotoxicity in tumor cells, revealed by CCK-8
AU - Kano, Arihiro
AU - Kamita, Moses Kibunja
AU - Iwasaki, Takuma
AU - Shindo, Mitsuru
PY - 2017/9
Y1 - 2017/9
N2 - Bongkrekic acid (BKA) is shown to inhibit adenine nucleotide translocator in the mitochondrial inner membrane, which is bifunctional and facilitates ATP/ADP exchange and permeability transition pore opening in mitochondria. We demonstrate that BKA reduces the viability of tumor cells depending on the cell number in the culture. This reduction was first demonstrated by Cell Counting Kit-8 (CCK-8), which indicates cellular viabilities based on the reduction of a formazan dye by cellular NADH. Reduced viability was not seen in normal cells. The glycolysis inhibitor 2-deoxyglucose enhanced the effect of BKA. BKA is thought to work as a mitochondrial inhibitor; however, its action is different from mitochondrial uncouplers. We further demonstrate that BKA suppresses autophagy induction, directing cells to death in nutrient-depleted conditions. Together these data indicate that BKA is a new type of mitochondrial inhibitor that does not disrupt the mitochondrial membrane and that is thus used together with a glycolysis inhibitor as an anticancer agent.
AB - Bongkrekic acid (BKA) is shown to inhibit adenine nucleotide translocator in the mitochondrial inner membrane, which is bifunctional and facilitates ATP/ADP exchange and permeability transition pore opening in mitochondria. We demonstrate that BKA reduces the viability of tumor cells depending on the cell number in the culture. This reduction was first demonstrated by Cell Counting Kit-8 (CCK-8), which indicates cellular viabilities based on the reduction of a formazan dye by cellular NADH. Reduced viability was not seen in normal cells. The glycolysis inhibitor 2-deoxyglucose enhanced the effect of BKA. BKA is thought to work as a mitochondrial inhibitor; however, its action is different from mitochondrial uncouplers. We further demonstrate that BKA suppresses autophagy induction, directing cells to death in nutrient-depleted conditions. Together these data indicate that BKA is a new type of mitochondrial inhibitor that does not disrupt the mitochondrial membrane and that is thus used together with a glycolysis inhibitor as an anticancer agent.
UR - http://www.scopus.com/inward/record.url?scp=85034649488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034649488&partnerID=8YFLogxK
U2 - 10.5109/1929661
DO - 10.5109/1929661
M3 - Article
AN - SCOPUS:85034649488
VL - 4
SP - 23
EP - 27
JO - Evergreen
JF - Evergreen
SN - 2189-0420
IS - 2-3
ER -