Bongkrekic acid induces selective cytotoxicity in tumor cells, revealed by CCK-8

Arihiro Kano, Moses Kibunja Kamita, Takuma Iwasaki, Mitsuru Shindo

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Bongkrekic acid (BKA) is shown to inhibit adenine nucleotide translocator in the mitochondrial inner membrane, which is bifunctional and facilitates ATP/ADP exchange and permeability transition pore opening in mitochondria. We demonstrate that BKA reduces the viability of tumor cells depending on the cell number in the culture. This reduction was first demonstrated by Cell Counting Kit-8 (CCK-8), which indicates cellular viabilities based on the reduction of a formazan dye by cellular NADH. Reduced viability was not seen in normal cells. The glycolysis inhibitor 2-deoxyglucose enhanced the effect of BKA. BKA is thought to work as a mitochondrial inhibitor; however, its action is different from mitochondrial uncouplers. We further demonstrate that BKA suppresses autophagy induction, directing cells to death in nutrient-depleted conditions. Together these data indicate that BKA is a new type of mitochondrial inhibitor that does not disrupt the mitochondrial membrane and that is thus used together with a glycolysis inhibitor as an anticancer agent.

元の言語英語
ページ(範囲)23-27
ページ数5
ジャーナルEvergreen
4
発行部数2-3
DOI
出版物ステータス出版済み - 9 2017

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Bongkrekic Acid
Cytotoxicity
tumor
Tumors
Cells
Acids
inhibitor
acid
viability
Formazans
membrane
Membranes
Administrative data processing
Mitochondria
Adenine Nucleotides
Adenosinetriphosphate
Deoxyglucose
mitochondrion
Nucleotides
Cell culture

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Surfaces, Coatings and Films
  • Management, Monitoring, Policy and Law

これを引用

Bongkrekic acid induces selective cytotoxicity in tumor cells, revealed by CCK-8. / Kano, Arihiro; Kamita, Moses Kibunja; Iwasaki, Takuma; Shindo, Mitsuru.

:: Evergreen, 巻 4, 番号 2-3, 09.2017, p. 23-27.

研究成果: ジャーナルへの寄稿記事

Kano, Arihiro ; Kamita, Moses Kibunja ; Iwasaki, Takuma ; Shindo, Mitsuru. / Bongkrekic acid induces selective cytotoxicity in tumor cells, revealed by CCK-8. :: Evergreen. 2017 ; 巻 4, 番号 2-3. pp. 23-27.
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