Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis

Taichi Koshiba, Masaru Kobayashi, Ken Matsuoka, Toru Fujiwara, Toru Matoh

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.

元の言語英語
ページ(範囲)189-194
ページ数6
ジャーナルSoil Science and Plant Nutrition
59
発行部数2
DOI
出版物ステータス出版済み - 4 1 2013

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boron
tobacco
B-lymphocytes
nutrition
damage
borate
ester
borates
tetrazolium
cells
Nicotiana tabacum
pectins
cell death
esters
cell walls
analysis
defence
catastrophe

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

これを引用

Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis. / Koshiba, Taichi; Kobayashi, Masaru; Matsuoka, Ken; Fujiwara, Toru; Matoh, Toru.

:: Soil Science and Plant Nutrition, 巻 59, 番号 2, 01.04.2013, p. 189-194.

研究成果: ジャーナルへの寄稿記事

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AU - Matoh, Toru

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