抄録
In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 189-194 |
| ページ数 | 6 |
| ジャーナル | Soil Science and Plant Nutrition |
| 巻 | 59 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 4 1 2013 |
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All Science Journal Classification (ASJC) codes
- Soil Science
- Plant Science
これを引用
Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis. / Koshiba, Taichi; Kobayashi, Masaru; Matsuoka, Ken; Fujiwara, Toru; Matoh, Toru.
：: Soil Science and Plant Nutrition, 巻 59, 番号 2, 01.04.2013, p. 189-194.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis
AU - Koshiba, Taichi
AU - Kobayashi, Masaru
AU - Matsuoka, Ken
AU - Fujiwara, Toru
AU - Matoh, Toru
PY - 2013/4/1
Y1 - 2013/4/1
N2 - In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
AB - In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
UR - http://www.scopus.com/inward/record.url?scp=84878438604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878438604&partnerID=8YFLogxK
U2 - 10.1080/00380768.2013.763383
DO - 10.1080/00380768.2013.763383
M3 - Article
AN - SCOPUS:84878438604
VL - 59
SP - 189
EP - 194
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
SN - 0038-0768
IS - 2
ER -