TY - JOUR
T1 - Boron nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of metabolic dysfunction in cells deprived of boron as revealed by microarray analysis
AU - Koshiba, Taichi
AU - Kobayashi, Masaru
AU - Matsuoka, Ken
AU - Fujiwara, Toru
AU - Matoh, Toru
N1 - Funding Information:
We thank T. Narisawa and M. Sasaki in RIKEN Plant Science Center for microarray data collection. The work was supported in part by a Grant-in-Aid for Scientific Research on Innovative Areas (No. 23119509 to MK) from The Ministry of Education, Culture, Sports, Science and Technology of Japan, and a Grant-in-Aid for Scientific Research (C) from the Japan Society for Promotion of Science (No. 20580062 to MK).
PY - 2013/4
Y1 - 2013/4
N2 - In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
AB - In plants, boron (B) works to build the cell wall structure by forming borate ester cross-links with pectin, and the deficiency of this element brings about oxidative damage and cell death. However, the process via which B deficiency leads to such catastrophe has not been well understood. In this study, we investigated the temporal changes in transcript profiles of B-deprived cultured tobacco (Nicotiana tabacum L.) BY-2 cells using cDNA microarray. The results suggest that the cells deprived of B up-regulated their antioxidative defense system to cope with the stress condition. However, the adaptive responses seemed to have declined within the first 12 h of deprivation. Tetrazolium reducing activity of B-deprived cells was lower than that of control cells at 12 h. These results together suggest that cellular function in B-deprived cells was seriously damaged as early as 12 h after deprivation. The rapidity of the onset of B deprivation-induced damages suggests the importance of the pectic network covalently cross-linked with B.
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U2 - 10.1080/00380768.2013.763383
DO - 10.1080/00380768.2013.763383
M3 - Article
AN - SCOPUS:84878438604
VL - 59
SP - 189
EP - 194
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
SN - 0038-0768
IS - 2
ER -