Bovine milk antibodies against cell surface protein antigen PAc- glucosyltransferase fusion protein suppress cell adhesion and alter glucan synthesis of Streptococcus mutans

Takahiko Oho, Yoshihiro Shimazaki, Morihide Mitoma, Mamiko Yoshimura, Yoshihisa Yamashita, Kaoru Okano, Yutaka Nakano, Hiroko Kawagoe, Masataka Fukuyama, Noboru Fujihara, Toshihiko Koga

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

Cell surface protein antigen (PAc) and glucosyltransferases (GTF) produced by Streptococcus mutans are considered major colonization factors of the organism, and the inhibition of these factors is thought to prevent dental caries. In this study, 8-mo-old pregnant Holstein cows were immunized with fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region (PAcA) of PAc with the glucan binding (GB) domain of GTF-I, an enzyme catalyzing the synthesis of water-insoluble glucan from sucrose. High titers of immunoglobulin antibodies specific for the fusion protein were found in normal milk after reimmunization, and they persisted for ~3 mo. The immunoglobulin G (IgG) antibodies against PAcA-GB were purified from immunized milk. The antibodies significantly inhibited the adhesion of S. mutans cells to saliva-coated hydroxyapatite beads. IgG antibodies purified from immunized milk also inhibited total glucan synthesis by cell-associated GTF preparation and GTF-I from S. mutans. The immunized milk may be useful as a means of passive immunization for the prevention of dental caries in humans.

本文言語英語
ページ(範囲)1836-1841
ページ数6
ジャーナルJournal of Nutrition
129
10
DOI
出版ステータス出版済み - 1 1 1999

All Science Journal Classification (ASJC) codes

  • 医学(その他)
  • 栄養および糖尿病

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