Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida; Alla Salmina; Minako Hashii; Shigeru Yokoyama; Jia Sheng Zhang; Mami Noda; Zen Guo Zhong; Duo Jin

doi:10.1016/j.febslet.2006.07.084

Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida, Alla Salmina, Minako Hashii, Shigeru Yokoyama, Jia Sheng Zhang, Mami Noda, Zen Guo Zhong, Duo Jin

臨床薬学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

4 被引用数 (Scopus)

抄録

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [³H] cyclic ADP-ribose (cADPR) formation from [³H] NAD⁺. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD⁺ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B₂ BK receptors.

本文言語	英語
ページ（範囲）	4857-4860
ページ数	4
ジャーナル	FEBS Letters
巻	580
号	20
DOI	https://doi.org/10.1016/j.febslet.2006.07.084
出版ステータス	出版済み - 9月 4 2006

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2006.07.084

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引用スタイル

Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells : Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose. / Higashida, Haruhiro; Salmina, Alla; Hashii, Minako; Yokoyama, Shigeru; Zhang, Jia Sheng; Noda, Mami; Zhong, Zen Guo; Jin, Duo.

In: FEBS Letters, Vol. 580, No. 20, 04.09.2006, p. 4857-4860.

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

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abstract = "ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.",

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AU - Higashida, Haruhiro

AU - Salmina, Alla

AU - Hashii, Minako

AU - Yokoyama, Shigeru

AU - Zhang, Jia Sheng

AU - Noda, Mami

AU - Zhong, Zen Guo

AU - Jin, Duo

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