Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida; Alla Salmina; Minako Hashii; Shigeru Yokoyama; Jia Sheng Zhang; Mami Noda; Zen Guo Zhong; Duo Jin

doi:10.1016/j.febslet.2006.07.084

Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

Haruhiro Higashida, Alla Salmina, Minako Hashii, Shigeru Yokoyama, Jia Sheng Zhang, Mami Noda, Zen Guo Zhong, Duo Jin

臨床薬学

研究成果: Contribution to journal › Article › 査読

3 被引用数 (Scopus)

抄録

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [³H] cyclic ADP-ribose (cADPR) formation from [³H] NAD⁺. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD⁺ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B₂ BK receptors.

本文言語	英語
ページ（範囲）	4857-4860
ページ数	4
ジャーナル	FEBS Letters
巻	580
号	20
DOI	https://doi.org/10.1016/j.febslet.2006.07.084
出版ステータス	出版済み - 9 4 2006

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

文献へのアクセス

10.1016/j.febslet.2006.07.084

他のファイルとリンク

フィンガープリント「Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells : Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose. / Higashida, Haruhiro; Salmina, Alla; Hashii, Minako; Yokoyama, Shigeru; Zhang, Jia Sheng; Noda, Mami; Zhong, Zen Guo; Jin, Duo.

In: FEBS Letters, Vol. 580, No. 20, 04.09.2006, p. 4857-4860.

研究成果: Contribution to journal › Article › 査読

@article{e7abb2ba6079477b8c4e882c331b5fb4,

title = "Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose",

abstract = "ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.",

author = "Haruhiro Higashida and Alla Salmina and Minako Hashii and Shigeru Yokoyama and Zhang, {Jia Sheng} and Mami Noda and Zhong, {Zen Guo} and Duo Jin",

year = "2006",

month = sep,

day = "4",

doi = "10.1016/j.febslet.2006.07.084",

language = "English",

volume = "580",

pages = "4857--4860",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "20",

}

TY - JOUR

T1 - Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells

T2 - Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose

AU - Higashida, Haruhiro

AU - Salmina, Alla

AU - Hashii, Minako

AU - Yokoyama, Shigeru

AU - Zhang, Jia Sheng

AU - Noda, Mami

AU - Zhong, Zen Guo

AU - Jin, Duo

PY - 2006/9/4

Y1 - 2006/9/4

N2 - ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

AB - ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

UR - http://www.scopus.com/inward/record.url?scp=33747839493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747839493&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.07.084

DO - 10.1016/j.febslet.2006.07.084

M3 - Article

C2 - 16905135

AN - SCOPUS:33747839493

VL - 580

SP - 4857

EP - 4860

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 20

ER -