Brain-transportable dipeptides across the blood-brain barrier in mice

Mitsuru Tanaka, Shinya Dohgu, Genki Komabayashi, Hayato Kiyohara, Fuyuko Takata, Yasufumi Kataoka, Takashi Nirasawa, Motohiro Maebuchi, Toshiro Matsui

研究成果: ジャーナルへの寄稿記事

抄録

Apart from nutrients required for the brain, there has been no report that naturally occurring peptides can cross the blood-brain barrier (BBB). The aim of this study was to identify the BBB-transportable peptides using in situ mouse perfusion experiments. Based on the structural features of Gly-N-methylated Gly (Gly-Sar), a reported BBB-transportable compound, 18 dipeptides were synthesized, and were perfused in the mouse brain for two minutes. Among the synthesized dipeptides, Gly-Sar, Gly-Pro, and Tyr-Pro were transported across the BBB with K i values of 7.60 ± 1.29, 3.49 ± 0.66, and 3.53 ± 0.74 µL/g·min, respectively, and accumulated in the mouse brain parenchyma. Additionally, using MALDI-MS/MS imaging analysis of Tyr-Pro-perfused brain, we provide evidence for Tyr-Pro accumulation in the hippocampus, hypothalamus, striatum, cerebral cortex, and cerebellum of mouse brain.

元の言語英語
記事番号5769
ジャーナルScientific reports
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2019

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Dipeptides
Blood-Brain Barrier
tyrosyl-proline
Brain
Peptides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cerebral Cortex
Cerebellum
Hypothalamus
Hippocampus
Perfusion
Food

All Science Journal Classification (ASJC) codes

  • General

これを引用

Brain-transportable dipeptides across the blood-brain barrier in mice. / Tanaka, Mitsuru; Dohgu, Shinya; Komabayashi, Genki; Kiyohara, Hayato; Takata, Fuyuko; Kataoka, Yasufumi; Nirasawa, Takashi; Maebuchi, Motohiro; Matsui, Toshiro.

:: Scientific reports, 巻 9, 番号 1, 5769, 01.12.2019.

研究成果: ジャーナルへの寄稿記事

Tanaka, M, Dohgu, S, Komabayashi, G, Kiyohara, H, Takata, F, Kataoka, Y, Nirasawa, T, Maebuchi, M & Matsui, T 2019, 'Brain-transportable dipeptides across the blood-brain barrier in mice', Scientific reports, 巻. 9, 番号 1, 5769. https://doi.org/10.1038/s41598-019-42099-9
Tanaka M, Dohgu S, Komabayashi G, Kiyohara H, Takata F, Kataoka Y その他. Brain-transportable dipeptides across the blood-brain barrier in mice. Scientific reports. 2019 12 1;9(1). 5769. https://doi.org/10.1038/s41598-019-42099-9
Tanaka, Mitsuru ; Dohgu, Shinya ; Komabayashi, Genki ; Kiyohara, Hayato ; Takata, Fuyuko ; Kataoka, Yasufumi ; Nirasawa, Takashi ; Maebuchi, Motohiro ; Matsui, Toshiro. / Brain-transportable dipeptides across the blood-brain barrier in mice. :: Scientific reports. 2019 ; 巻 9, 番号 1.
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