Brassinosteroid involvement in arabidopsis thaliana stomatal opening

Shin Ichiro Inoue, Nozomi Iwashita, Yohei Takahashi, Eiji Gotoh, Eiji Okuma, Maki Hayashi, Ryohei Tabata, Atsushi Takemiya, Yoshiyuki Murata, Michio Doi, Toshinori Kinoshita, Ken Ichiro Shimazaki

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Stomata within the plant epidermis regulate CO 2 uptake for photosynthesis and water loss through transpiration. Stomatal opening in Arabidopsis thaliana is determined by various factors, including blue light as a signal and multiple phytohormones. Plasma membrane transporters, including H + -ATPase, K + channels and anion channels in guard cells, mediate these processes, and the activities and expression levels of these components determine stomatal aperture. However, the regulatory mechanisms involved in these processes are not fully understood. In this study, we used infrared thermography to isolate a mutant defective in stomatal opening in response to light. The causative mutation was identified as an allele of the brassinosteroid (BR) biosynthetic mutant dwarf5. Guard cells from this mutant exhibited normal H + -ATPase activity in response to blue light, but showed reduced K + accumulation and inward-rectifying K + (K + in) channel activity as a consequence of decreased expression of major K + in channel genes. Consistent with these results, another BR biosynthetic mutant, det2-1, and a BR receptor mutant, bri1-6, exhibited reduced blue light-dependent stomatal opening. Furthermore, application of BR to the hydroponic culture medium completely restored stomatal opening in dwarf5 and det2-1 but not in bri1-6. However, application of BR to the epidermis of dwarf5 did not restore stomatal response. From these results, we conclude that endogenous BR acts in a long-term manner and is required in guard cells with the ability to open stomata in response to light, probably through regulation of K + in channel activity.

元の言語英語
ページ(範囲)1048-1058
ページ数11
ジャーナルPlant and Cell Physiology
58
発行部数6
DOI
出版物ステータス出版済み - 6 1 2017

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Brassinosteroids
brassinosteroids
Arabidopsis
Arabidopsis thaliana
potassium channels
guard cells
Light
mutants
blue light
H-transporting ATP synthase
Proton-Translocating ATPases
epidermis (plant)
stomata
Plant Epidermis
Hydroponics
thermography
Plant Growth Regulators
Membrane Transport Proteins
Photosynthesis
Carbon Monoxide

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

これを引用

Inoue, S. I., Iwashita, N., Takahashi, Y., Gotoh, E., Okuma, E., Hayashi, M., ... Shimazaki, K. I. (2017). Brassinosteroid involvement in arabidopsis thaliana stomatal opening. Plant and Cell Physiology, 58(6), 1048-1058. https://doi.org/10.1093/pcp/pcx049

Brassinosteroid involvement in arabidopsis thaliana stomatal opening. / Inoue, Shin Ichiro; Iwashita, Nozomi; Takahashi, Yohei; Gotoh, Eiji; Okuma, Eiji; Hayashi, Maki; Tabata, Ryohei; Takemiya, Atsushi; Murata, Yoshiyuki; Doi, Michio; Kinoshita, Toshinori; Shimazaki, Ken Ichiro.

:: Plant and Cell Physiology, 巻 58, 番号 6, 01.06.2017, p. 1048-1058.

研究成果: ジャーナルへの寄稿記事

Inoue, SI, Iwashita, N, Takahashi, Y, Gotoh, E, Okuma, E, Hayashi, M, Tabata, R, Takemiya, A, Murata, Y, Doi, M, Kinoshita, T & Shimazaki, KI 2017, 'Brassinosteroid involvement in arabidopsis thaliana stomatal opening', Plant and Cell Physiology, 巻. 58, 番号 6, pp. 1048-1058. https://doi.org/10.1093/pcp/pcx049
Inoue SI, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M その他. Brassinosteroid involvement in arabidopsis thaliana stomatal opening. Plant and Cell Physiology. 2017 6 1;58(6):1048-1058. https://doi.org/10.1093/pcp/pcx049
Inoue, Shin Ichiro ; Iwashita, Nozomi ; Takahashi, Yohei ; Gotoh, Eiji ; Okuma, Eiji ; Hayashi, Maki ; Tabata, Ryohei ; Takemiya, Atsushi ; Murata, Yoshiyuki ; Doi, Michio ; Kinoshita, Toshinori ; Shimazaki, Ken Ichiro. / Brassinosteroid involvement in arabidopsis thaliana stomatal opening. :: Plant and Cell Physiology. 2017 ; 巻 58, 番号 6. pp. 1048-1058.
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AU - Okuma, Eiji

AU - Hayashi, Maki

AU - Tabata, Ryohei

AU - Takemiya, Atsushi

AU - Murata, Yoshiyuki

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AU - Kinoshita, Toshinori

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