Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF- B activation in microglia

Zhou Wu, Aiqin Zhu, Fumiko Takayama, Ryo Okada, Yicong Liu, Yuka Harada, Shizheng Wu, Hiroshi Nakanishi

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Hypoxia has been recently proposed as a neuroinflammatogen, which drives microglia to produce proinflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α; (TNF-α;), and IL-6. Considering the fact that propolis has hepatoprotective, antitumor, antioxidative, and anti-inflammatory effects, propolis may have protective effects against the hypoxia-induced neuroinflammatory responses. In this study, propolis (50 g/mL) was found to significantly inhibit the hypoxia-induced cytotoxicity and the release of proinflammatory cytokines, including IL-1β, TNF-α;, and IL-6, by MG6 microglia following hypoxic exposure (1% O 2, 24 h). Furthermore, propolis significantly inhibited the hypoxia-induced generation of reactive oxygen species (ROS) from mitochondria and the activation of nuclear factor-B (NF-B) in microglia. Moreover, systemic treatment with propolis (8.33 mg/kg, 2 times/day, i.p.) for 7 days significantly suppressed the microglial expression of IL-1β, TNF-α;, IL-6, and 8-oxo-deoxyguanosine, a biomarker for oxidative damaged DNA, in the somatosensory cortex of mice subjected to hypoxia exposure (10% O2, 4 h). These observations indicate that propolis suppresses the hypoxia-induced neuroinflammatory responses through inhibition of the NF-B activation in microglia. Furthermore, increased generation of ROS from the mitochondria is responsible for the NF-B activation. Therefore, propolis may be beneficial in preventing hypoxia-induced neuroinflammation.

元の言語英語
記事番号906726
ジャーナルOxidative medicine and cellular longevity
DOI
出版物ステータス出版済み - 9 3 2013

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Propolis
Microglia
Chemical activation
Interleukin-1
Interleukin-6
Mitochondria
Reactive Oxygen Species
Cytokines
Deoxyguanosine
Somatosensory Cortex
Biomarkers
Cytotoxicity
Hypoxia
Interleukin-8
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
DNA

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Ageing
  • Biochemistry
  • Medicine(all)

これを引用

Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF- B activation in microglia. / Wu, Zhou; Zhu, Aiqin; Takayama, Fumiko; Okada, Ryo; Liu, Yicong; Harada, Yuka; Wu, Shizheng; Nakanishi, Hiroshi.

:: Oxidative medicine and cellular longevity, 03.09.2013.

研究成果: ジャーナルへの寄稿記事

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abstract = "Hypoxia has been recently proposed as a neuroinflammatogen, which drives microglia to produce proinflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α; (TNF-α;), and IL-6. Considering the fact that propolis has hepatoprotective, antitumor, antioxidative, and anti-inflammatory effects, propolis may have protective effects against the hypoxia-induced neuroinflammatory responses. In this study, propolis (50 g/mL) was found to significantly inhibit the hypoxia-induced cytotoxicity and the release of proinflammatory cytokines, including IL-1β, TNF-α;, and IL-6, by MG6 microglia following hypoxic exposure (1{\%} O 2, 24 h). Furthermore, propolis significantly inhibited the hypoxia-induced generation of reactive oxygen species (ROS) from mitochondria and the activation of nuclear factor-B (NF-B) in microglia. Moreover, systemic treatment with propolis (8.33 mg/kg, 2 times/day, i.p.) for 7 days significantly suppressed the microglial expression of IL-1β, TNF-α;, IL-6, and 8-oxo-deoxyguanosine, a biomarker for oxidative damaged DNA, in the somatosensory cortex of mice subjected to hypoxia exposure (10{\%} O2, 4 h). These observations indicate that propolis suppresses the hypoxia-induced neuroinflammatory responses through inhibition of the NF-B activation in microglia. Furthermore, increased generation of ROS from the mitochondria is responsible for the NF-B activation. Therefore, propolis may be beneficial in preventing hypoxia-induced neuroinflammation.",
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AU - Liu, Yicong

AU - Harada, Yuka

AU - Wu, Shizheng

AU - Nakanishi, Hiroshi

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