TY - JOUR
T1 - Breakdown of Bruch's Membrane after Subretinal Injection of Vitreous
T2 - Role of Cellular Processes
AU - Zhu, Zhi Ren
AU - Goodnight, Randi
AU - Ishibashi, Tatsuro
AU - Sorgente, Nino
AU - Ogden, Thomas E.
AU - Ryan, Stephen J.
N1 - Funding Information:
Supported in part by a grant from Research to Prevent Blindness, Inc, New York and grants EY01545 and EY03040 from the National Eye Institute, NIH, Bethesda, Maryland.
PY - 1988
Y1 - 1988
N2 - It was recently shown that the injection of autologous vitreous beneath the retina of rabbits leads to retinal degeneration, subretinal cellular proliferation and neovascularization. The current study, using electron microscopy, was designed to determine the cellular processes involved in the breakdown of Bruch's membrane in this model. Bruch's membrane was not mechanically damaged by the injection and appeared intact for the first 1 to 2 days after injection. Subsequently, numerous breaks in Bruch's membrane were found associated with invasion of macrophages and fibroblasts; in addition, budding and penetration of retinal pigment epithelial (RPE) and choroidal endothelial cells into Bruch's membrane were noted. Although it was not proven that these cells, per se, were responsible for the breaks, that these cells actively penetrate Bruch's membrane is a reasonable hypothesis.
AB - It was recently shown that the injection of autologous vitreous beneath the retina of rabbits leads to retinal degeneration, subretinal cellular proliferation and neovascularization. The current study, using electron microscopy, was designed to determine the cellular processes involved in the breakdown of Bruch's membrane in this model. Bruch's membrane was not mechanically damaged by the injection and appeared intact for the first 1 to 2 days after injection. Subsequently, numerous breaks in Bruch's membrane were found associated with invasion of macrophages and fibroblasts; in addition, budding and penetration of retinal pigment epithelial (RPE) and choroidal endothelial cells into Bruch's membrane were noted. Although it was not proven that these cells, per se, were responsible for the breaks, that these cells actively penetrate Bruch's membrane is a reasonable hypothesis.
UR - http://www.scopus.com/inward/record.url?scp=0023927345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023927345&partnerID=8YFLogxK
U2 - 10.1016/S0161-6420(88)33074-5
DO - 10.1016/S0161-6420(88)33074-5
M3 - Article
C2 - 3174042
AN - SCOPUS:0023927345
VL - 95
SP - 925
EP - 929
JO - Ophthalmology
JF - Ophthalmology
SN - 0161-6420
IS - 7
ER -