Brefeldin A inhibits the targeting of cathepsin D and cathepsin H to lysosomes in rat hepatocytes

Kimimitsu Oda, Yukio Nishimura

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Effect of brefeldin A on the transport of lysosomal acid hydrolases (cathepsins D and H) was investigated in primary cultured rat hepatocytes. Both cathepsins were synthesized as proenzymes and progressively converted to mature enzymes in the control cells. However, BFA strongly inhibited the appearance of the mature enzymes in the cells in a dose dependent manner, suggesting that transport of newly synthesized lysosomal enzymes from the endoplasmic reticulum to lysosomes is blocked by the drug. The inhibitory effect by brefeldin A was reversible. Upon recovery from brefeldin A-intoxication, procathepsin D was effectively targeted into lysosomes, whereas a substantial amount of procathepsin H was found to be missorted, resulting in its secretion into the culture medium.

元の言語英語
ページ(範囲)220-225
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
163
発行部数1
DOI
出版物ステータス出版済み - 8 30 1989

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Cathepsin H
Brefeldin A
Cathepsin D
Lysosomes
Rats
Hepatocytes
Enzymes
Cathepsins
Enzyme Precursors
Hydrolases
Endoplasmic Reticulum
Culture Media
Recovery
Acids
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Brefeldin A inhibits the targeting of cathepsin D and cathepsin H to lysosomes in rat hepatocytes. / Oda, Kimimitsu; Nishimura, Yukio.

:: Biochemical and Biophysical Research Communications, 巻 163, 番号 1, 30.08.1989, p. 220-225.

研究成果: ジャーナルへの寄稿記事

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