Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The stochastic multicolor labeling method ‘Brainbow’ is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architecture of individual neurons. Using Tetbow, we were able to visualize the axonal projection patterns of individual mitral/tufted cells along several millimeters in the mouse olfactory system. We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. This was useful in expanding the repertoire of the fluorescence labels and the applications of the Tetbow system. Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution.

元の言語英語
記事番号e40350
ジャーナルeLife
7
DOI
出版物ステータス出版済み - 11 1 2018

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Labeling
Fluorescence
Neurons
Networks (circuits)
Labels
Tetracycline
Microscopy
Proteins
Plasmids
Fluorophores
Viruses
Light
Optical microscopy
Tissue

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. / Sakaguchi, Richi; Leiwe, Marcus Nathanael; Imai, Takeshi.

:: eLife, 巻 7, e40350, 01.11.2018.

研究成果: ジャーナルへの寄稿記事

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