BspR/BtrA, an Anti-σ factor, regulates the ability of Bordetella bronchiseptica to cause cough in rats

Keiji Nakamura, Noriko Shinoda, Yukihiro Hiramatsu, Shinya Ohnishi, Shigeki Kamitani, Yoshitoshi Ogura, Tetsuya Hayashi, Yasuhiko Horiguchi

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections, many of which are characterized by coughing of the infected hosts. The pathogenesis of the coughing remains to be analyzed, mainly because there were no convenient infection models of small animals that replicate coughing after Bordetella infection. Here, we present a coughing model of rats infected with B. bronchiseptica. Rats, which are one of natural hosts of B. bronchiseptica, were readily infected with the organisms and showed frequent coughing. B. pertussis also caused coughing in rats, which is consistent with previous reports, but the cough response was less apparent than the B. bronchiseptica-induced cough. By using the rat model, we demonstrated that adenylate cyclase toxin, dermonecrotic toxin, and the type III secretion system are not involved in cough production, but BspR/BtrA (different names for the same protein), an anti-σ factor, regulates the production of unknown factor(s) to cause coughing. Rat coughing was observed by inoculation of not only the living bacteria but also the bacterial lysates. Infection with bspR (btrA)-deficient strains caused significantly less frequent coughing than the wild type; however, intranasal inoculation of the lysates from a bspR (btrA)-deficient strain caused coughing similarly to the wild type, suggesting that BspR/BtrA regulates the production of the cough factor(s) only when the bacteria colonize host bodies. Moreover, the cough factor(s) was found to be heat labile and produced by B. bronchiseptica in the Bvg+ phase. We consider that our rat model provides insight into the pathogenesis of cough induced by the Bordetella infection.

本文言語英語
論文番号e00093-19
ジャーナルmSphere
4
2
DOI
出版ステータス出版済み - 3 1 2019

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学

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