Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines

Ryosuke Fujita, Ryusei Kuwata, Daisuke Kobayashi, Arlene Garcia Bertuso, Haruhiko Isawa, Kyoko Sawabe

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other virus, from a Mansonia sp. mosquito, is a new negevirus designated Bustos virus. Gene expression analysis of the Bustos virus revealed that infected cells contain viral subgenomic RNAs that probably include open reading frame (ORF) 2 or ORF3. Purified Bustos virus particles contained at least three proteins, and the major component (a probable major capsid protein) is encoded by ORF3. Bustos virus did not show infectivity in mammalian BHK-21 cells, suggesting that it is an insect-specific virus, like other known negeviruses.

本文言語英語
ページ(範囲)79-88
ページ数10
ジャーナルArchives of Virology
162
1
DOI
出版ステータス出版済み - 1 1 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • ウイルス学

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