Bystander-killing effect and cyclic induction of TNF-α gene under heat-inducible promoter gadd 153

Akira Ito, Masashige Shinkai, Isabelle Anne Bouhon, Hiroyuki Honda, Takeshi Kobayashi

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-a gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-a gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell line U251-SP. When the transfected cells were exposed to heat treatment, TNF-a gene expression was induced. The heated pGadTNF-transfected cells were co-cultured the nonheated pGadTNF-transfected cells as bystander cells. As a result, TNF-a gene expression in the bystander cells was observed. The level of TNF-a gene expression was further enhanced in the co-cultured cells. Due to this cyclic induction, a strong cytotoxic effect on the bystander cells was observed. Based on this bystander-killing effect, it was concluded that the transfection of the TNF-a gene under the control of the gadd 153 promoter into tumor cells combined with hyperthermia would be a potent tool for cancer therapy.

本文言語英語
ページ(範囲)437-441
ページ数5
ジャーナルJournal of Bioscience and Bioengineering
90
4
DOI
出版ステータス出版済み - 10 2000

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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