C-Terminal Identification of AD74, a Proteolytic Product of Enterococcus faecalis Aggregation Substance: Application of Liquid Chromatography/Mass Spectrometry

Jiro Nakayama, Hiroshi Watarai, Akira Isogai, Akinori Suzuki

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pADl is enhanced by the sex pheromone cADl, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADl-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511.

本文言語英語
ページ(範囲)127-131
ページ数5
ジャーナルBioscience, biotechnology, and biochemistry
56
1
DOI
出版ステータス出版済み - 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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