C18:3-GM1a induces apoptosis in Neuro2a cells: Enzymatic remodeling of fatty acyl chains of glycosphingolipids

Tetsuto Nakagawa, Akio Morotomi, Motohiro Tani, Noriyuki Sueyoshi, Hironobu Komori, Makoto Ito

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

GM1a [Galβ1-3GalNAcβ1-4(NeuAcα-3)Galβ 1-4Glcβ1-1Cer] is known to support and protect neuronal functions. However, we report that α-linolenic acid-containing GM1a (C18:3-GM1a), which was prepared using the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase, induced apoptosis in neuronal cells. Intranucleosomal DNA fragmentation, chromatin condensation, and caspase activation, all typical features of apoptosis, were observed when mouse neuroblastoma Neuro2a cells were cultured with C18:3-GM1a but not GM1a containing stearic acid (C18:0) or oleic acid (C18:1). The phenotype of Neuro2a cells induced by C18:3-GM1a was similar to that evoked by lyso-GM1a. However, lyso-GM1a caused a complete disruption of lipid microdomains of Neuro2a cells and hemolysis of sheep erythrocytes, whereas C18:3-GM1a did neither. C18:3-GM1a, but not lyso-GM1a, was found to be abundant in lipid microdomains after the removal of loosely bound GM1a by BSA. The activation of stress-activated protein kinase/c-Jun N-terminal kinase in Neuro2a cells was observed with lyso-GM1a but not C18:3-GM1a. These results indicate that the mechanism of apoptosis induced by C18:3-GM1a is distinct from that caused by lyso-GM1a. This study also clearly shows that fatty acid composition of gangliosides significantly affected their pharmacological activities when added to the cell cultures and suggests why naturally occurring gangliosides do not possess polyunsaturated fatty acids as a major constituent.

本文言語英語
ページ(範囲)1103-1112
ページ数10
ジャーナルJournal of Lipid Research
46
6
DOI
出版ステータス出版済み - 2005

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 内分泌学
  • 細胞生物学

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