Ca2+ dependence of inositol 1,4,5-trisphosphate 3-kinase activity in the cytosol fraction of pig coronary artery

Koji Yamaguchi, Masato Hirata, Toyohiro Ishimatsu, Hirosi Kuriyama

研究成果: ジャーナルへの寄稿記事

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1. The activity of inositol 1,4,5-trisphosphate 3-kinase in subcellular fractions of smooth muscles of the pig coronary artery was examined. 2. Incubation of [3H]inositol 1,4,5-trisphosphate (IP3) with muscle homogenates produced more polar 3H-radioactivity (probably as inositol 1,3,4,5-tetrakisphosphate, IP4) than IP3, in the Mg2+- and ATP-dependent manner, thereby indicating the presence of IP3 3-kinase activity in homogenates of the muscle. 3. Most of the kinase activity was present in the cytosol fraction. The enzyme activity was reversibly activated by Ca2+ with a half-maximal effective concentration of 2.5 × 10-7 M. 4. The calmodulin antagonists, W-7 and chlorpromazine inhibited the Ca2+-activated enzyme activity.

元の言語英語
ページ(範囲)163-166
ページ数4
ジャーナルComparative Biochemistry and Physiology. Part C, Comparative
92
発行部数1
DOI
出版物ステータス出版済み - 1989

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

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