Calorie restriction initiated at a young age activates the Akt/PKCζ/λ-Glut4 pathway in rat white adipose tissue in an insulin-independent manner

Seongjoon Park, Toshimitsu Komatsu, Hiroko Hayashi, Haruyoshi Yamaza, Takuya Chiba, Yoshikazu Higami, Kazunao Kuramoto, Isao Shimokawa

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Calorie restriction (CR) may exert an anti-aging effect through a metabolic adaptation to limited energy intake. The present study investigated the effect of CR on insulin signaling in response to glucose load in the epididymal adipose tissue of male F344 rats at 7 and 22 months of age. Young and middle-aged rats were fed ad libitum (AL) or 30% CR diets for 4 months, underwent glucose tolerance tests and were sacrificed 15 min after an intraperitoneal glucose or saline injection to evaluate glucose-stimulated insulin response and subsequent activation of insulin signaling molecules in the adipose tissue. In the 7- and 22- month AL groups, glucose administration increased serum insulin levels and also increased phosphorylated (p) levels of the insulin receptor (IR), v-akt murine thymoma viral oncogene homolog (Akt), protein kinase C (PKC) ζ/λ and the membrane fraction of glucose transporter 4 (mGlut4). In contrast, in the 7-month CR group, p-Akt, p-PKCζ/λ and mGlut4 levels were upregulated without glucose stimulation; the glucose load augmented the p-IR level but there was no additional activation of the downstream molecules. In the 22-month CR group, these unexpected findings were not observed. In summary, basal levels of insulin signaling molecules such as p-Akt, p-PKCζ/λ, and mGlut4 were significantly increased with a low insulin response in the 7-month CR group. The present results suggest the presence of an age-specific insulin-independent mechanism that is induced by CR to regulate energy metabolism in white adipose tissue.

元の言語英語
ページ(範囲)293-302
ページ数10
ジャーナルAge
30
発行部数4
DOI
出版物ステータス出版済み - 12 1 2008
外部発表Yes

Fingerprint

White Adipose Tissue
Protein Kinase C
Insulin
Glucose
Facilitative Glucose Transport Proteins
Insulin Receptor
Membranes
Adipose Tissue
Thymoma
Inbred F344 Rats
Glucose Tolerance Test
Energy Intake
Oncogenes
Energy Metabolism
Diet
Injections
Serum

All Science Journal Classification (ASJC) codes

  • Ageing
  • Geriatrics and Gerontology

これを引用

Calorie restriction initiated at a young age activates the Akt/PKCζ/λ-Glut4 pathway in rat white adipose tissue in an insulin-independent manner. / Park, Seongjoon; Komatsu, Toshimitsu; Hayashi, Hiroko; Yamaza, Haruyoshi; Chiba, Takuya; Higami, Yoshikazu; Kuramoto, Kazunao; Shimokawa, Isao.

:: Age, 巻 30, 番号 4, 01.12.2008, p. 293-302.

研究成果: ジャーナルへの寄稿記事

Park, Seongjoon ; Komatsu, Toshimitsu ; Hayashi, Hiroko ; Yamaza, Haruyoshi ; Chiba, Takuya ; Higami, Yoshikazu ; Kuramoto, Kazunao ; Shimokawa, Isao. / Calorie restriction initiated at a young age activates the Akt/PKCζ/λ-Glut4 pathway in rat white adipose tissue in an insulin-independent manner. :: Age. 2008 ; 巻 30, 番号 4. pp. 293-302.
@article{75c070df1a23447b8bbb22a9b104d8f3,
title = "Calorie restriction initiated at a young age activates the Akt/PKCζ/λ-Glut4 pathway in rat white adipose tissue in an insulin-independent manner",
abstract = "Calorie restriction (CR) may exert an anti-aging effect through a metabolic adaptation to limited energy intake. The present study investigated the effect of CR on insulin signaling in response to glucose load in the epididymal adipose tissue of male F344 rats at 7 and 22 months of age. Young and middle-aged rats were fed ad libitum (AL) or 30{\%} CR diets for 4 months, underwent glucose tolerance tests and were sacrificed 15 min after an intraperitoneal glucose or saline injection to evaluate glucose-stimulated insulin response and subsequent activation of insulin signaling molecules in the adipose tissue. In the 7- and 22- month AL groups, glucose administration increased serum insulin levels and also increased phosphorylated (p) levels of the insulin receptor (IR), v-akt murine thymoma viral oncogene homolog (Akt), protein kinase C (PKC) ζ/λ and the membrane fraction of glucose transporter 4 (mGlut4). In contrast, in the 7-month CR group, p-Akt, p-PKCζ/λ and mGlut4 levels were upregulated without glucose stimulation; the glucose load augmented the p-IR level but there was no additional activation of the downstream molecules. In the 22-month CR group, these unexpected findings were not observed. In summary, basal levels of insulin signaling molecules such as p-Akt, p-PKCζ/λ, and mGlut4 were significantly increased with a low insulin response in the 7-month CR group. The present results suggest the presence of an age-specific insulin-independent mechanism that is induced by CR to regulate energy metabolism in white adipose tissue.",
author = "Seongjoon Park and Toshimitsu Komatsu and Hiroko Hayashi and Haruyoshi Yamaza and Takuya Chiba and Yoshikazu Higami and Kazunao Kuramoto and Isao Shimokawa",
year = "2008",
month = "12",
day = "1",
doi = "10.1007/s11357-008-9071-2",
language = "English",
volume = "30",
pages = "293--302",
journal = "GeroScience",
issn = "2509-2715",
publisher = "Springer International Publishing AG",
number = "4",

}

TY - JOUR

T1 - Calorie restriction initiated at a young age activates the Akt/PKCζ/λ-Glut4 pathway in rat white adipose tissue in an insulin-independent manner

AU - Park, Seongjoon

AU - Komatsu, Toshimitsu

AU - Hayashi, Hiroko

AU - Yamaza, Haruyoshi

AU - Chiba, Takuya

AU - Higami, Yoshikazu

AU - Kuramoto, Kazunao

AU - Shimokawa, Isao

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Calorie restriction (CR) may exert an anti-aging effect through a metabolic adaptation to limited energy intake. The present study investigated the effect of CR on insulin signaling in response to glucose load in the epididymal adipose tissue of male F344 rats at 7 and 22 months of age. Young and middle-aged rats were fed ad libitum (AL) or 30% CR diets for 4 months, underwent glucose tolerance tests and were sacrificed 15 min after an intraperitoneal glucose or saline injection to evaluate glucose-stimulated insulin response and subsequent activation of insulin signaling molecules in the adipose tissue. In the 7- and 22- month AL groups, glucose administration increased serum insulin levels and also increased phosphorylated (p) levels of the insulin receptor (IR), v-akt murine thymoma viral oncogene homolog (Akt), protein kinase C (PKC) ζ/λ and the membrane fraction of glucose transporter 4 (mGlut4). In contrast, in the 7-month CR group, p-Akt, p-PKCζ/λ and mGlut4 levels were upregulated without glucose stimulation; the glucose load augmented the p-IR level but there was no additional activation of the downstream molecules. In the 22-month CR group, these unexpected findings were not observed. In summary, basal levels of insulin signaling molecules such as p-Akt, p-PKCζ/λ, and mGlut4 were significantly increased with a low insulin response in the 7-month CR group. The present results suggest the presence of an age-specific insulin-independent mechanism that is induced by CR to regulate energy metabolism in white adipose tissue.

AB - Calorie restriction (CR) may exert an anti-aging effect through a metabolic adaptation to limited energy intake. The present study investigated the effect of CR on insulin signaling in response to glucose load in the epididymal adipose tissue of male F344 rats at 7 and 22 months of age. Young and middle-aged rats were fed ad libitum (AL) or 30% CR diets for 4 months, underwent glucose tolerance tests and were sacrificed 15 min after an intraperitoneal glucose or saline injection to evaluate glucose-stimulated insulin response and subsequent activation of insulin signaling molecules in the adipose tissue. In the 7- and 22- month AL groups, glucose administration increased serum insulin levels and also increased phosphorylated (p) levels of the insulin receptor (IR), v-akt murine thymoma viral oncogene homolog (Akt), protein kinase C (PKC) ζ/λ and the membrane fraction of glucose transporter 4 (mGlut4). In contrast, in the 7-month CR group, p-Akt, p-PKCζ/λ and mGlut4 levels were upregulated without glucose stimulation; the glucose load augmented the p-IR level but there was no additional activation of the downstream molecules. In the 22-month CR group, these unexpected findings were not observed. In summary, basal levels of insulin signaling molecules such as p-Akt, p-PKCζ/λ, and mGlut4 were significantly increased with a low insulin response in the 7-month CR group. The present results suggest the presence of an age-specific insulin-independent mechanism that is induced by CR to regulate energy metabolism in white adipose tissue.

UR - http://www.scopus.com/inward/record.url?scp=56749170729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56749170729&partnerID=8YFLogxK

U2 - 10.1007/s11357-008-9071-2

DO - 10.1007/s11357-008-9071-2

M3 - Article

C2 - 19424853

AN - SCOPUS:56749170729

VL - 30

SP - 293

EP - 302

JO - GeroScience

JF - GeroScience

SN - 2509-2715

IS - 4

ER -