CaMKII regulates the strength of the epithelial barrier

Ryo Shiomi, Kenta Shigetomi, Tetsuichiro Inai, Masami Sakai, Junichi Ikenouchi

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Epithelial cells define the boundary between the outside and the inside of our body by constructing the diffusion barrier. Tight junctions (TJs) of epithelial cells function as barriers against invasion of harmful microorganisms into the human body and free diffusion of water or ions from the body. Therefore, formation of TJs has to be strictly controlled in epithelial cells. However, the molecular mechanisms governing this regulation are largely unknown. In this study, we identified Ca2+ /calmodulin-dependent protein kinase II (CaMKII) as a regulator of the barrier function of TJs. CaMKII inhibition led to enlargement of TJ-areas and up-regulation of the barrier function. CaMKII inhibition induced excess TJ formation in part by the activation of AMP-activated protein kinase (AMPK) and subsequent phosphorylation of claudin-1. As up-regulation of epithelial barriers is essential for the prevention of chronic inflammatory diseases, the identification of CaMKII as a modulator of TJ function paves the way for the development of new drugs to treat these diseases.

元の言語英語
記事番号13262
ジャーナルScientific reports
5
DOI
出版物ステータス出版済み - 8 18 2015

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Tight Junctions
Epithelial Cells
Up-Regulation
Claudin-1
AMP-Activated Protein Kinases
Human Body
Chronic Disease
Phosphorylation
Ions
Water
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • General

これを引用

CaMKII regulates the strength of the epithelial barrier. / Shiomi, Ryo; Shigetomi, Kenta; Inai, Tetsuichiro; Sakai, Masami; Ikenouchi, Junichi.

:: Scientific reports, 巻 5, 13262, 18.08.2015.

研究成果: ジャーナルへの寄稿記事

Shiomi, Ryo ; Shigetomi, Kenta ; Inai, Tetsuichiro ; Sakai, Masami ; Ikenouchi, Junichi. / CaMKII regulates the strength of the epithelial barrier. :: Scientific reports. 2015 ; 巻 5.
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