Cancer cell separator using size-dependent filtration in microfluidic chip

Taisuke Masuda, Miyako Niimi, Hayao Nakanishi, Yoko Yamanishi, Fumihito Arai

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

We have developed a microfluidic chip that can separate gastric cancer cells from peritoneal washes. Gastric cancer cells tend to be agglomerated in the peritoneal wash, and are larger than the other cells and the soft tissue fragments. This paper presents a novel microfluidic chip to enrich low-concentrated cancer cells from the peritoneal wash by size-dependent cell filtration. This microfluidic chip has the advantage that it can separate agglomerated cells in high speed because larger objects are filtered by a gap in the microchannel. Two-step exposure was performed to fabricate a precise uneven channel for cell filtration. The main and shallow channels have heights of 100 μm and 8 μm, respectively. Furthermore, inserting fins in the microchannel that assist sheath flow enhanced continuous and robust separation against flow fluctuation in the microchannel. The microfluidic channel was designed to have high filtration efficiency and a high throughput while having a compact profile. We used patient's peritoneal washes to isolate cancer cells. As a result, we were able to enrich the cancer cells in peritoneal wash.

本文言語英語
ページ(範囲)245-251
ページ数7
ジャーナルSensors and Actuators, B: Chemical
185
DOI
出版ステータス出版済み - 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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