Carbon Dots Modification for Escherichia coli Detection: Variation of Colistin Sulphate Concentration

Suherman, NICKO AUDIO HARYANTO, ENDANG TRI WAHYUNI, MIFTAHUL ILMI, KINICHI MORITA, Yuji Oki

研究成果: ジャーナルへの寄稿記事

抄録

Escherichia coli bacteria widely recognize as a major treatment in water quality as diarrhea disease becoming one of leading causes of mortality worldwide. Carbon dots (CDs) modification study for detection of E. coli with the variation of colistin sulphate concentration has been conducted. The purpose of this research is to evaluate the effect of colistin sulphate concentration variation on fluorescence intensity for detecting E. coli bacteria in water samples. This study started by synthesizing CDs and CDs colistin variation concentrations of 1.0; 1.5; 2.0 and 2.5 mmol through carbonization of ammonium citrate and the addition of colistin. All precursors are characterized by using FT-IR. Morphological forms were evaluated with scanning emission microscopy (SEM) and transmission electron microscopy (TEM). CDs and CDs colistin solutions were measured their spectras with a UV-Vis spectrophotometer. Each of these CDs colistin was determined at the excitation wavelength ranges of 310-450 nm using a spectrofluorophotometer. Subsequently, CDs colistin was used for bacterial detection in samples with variation in E. coli bacteria concentration, thus analyzed by spectrofluorophotometer with an optimum excitation wavelength. The results showed that the highest intensity of fluorescence on CDs colistin occurred at the wavelength excitation of 360 nm. CDs colistin with the variation of colistin concentration 2.5 mmol revealed the highest fluorescence intensity. For the detection of E. coli, all CDs colistin produced the same trends of fluorescence spectra as the higher concentration of E. coli in the samples produced the higher intensity of emission.
元の言語英語
ページ(範囲)49-55
ページ数7
ジャーナルOriental Journal of Chemistry
35
発行部数1
DOI
出版物ステータス出版済み - 2019

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Colistin
Escherichia coli
Carbon
sulfate
carbon
fluorescence
Fluorescence
Bacteria
wavelength
bacterium
Wavelength
detection
Ultraviolet spectrophotometers
Water Purification
Water Quality
Carbonization
Transmission Electron Microscopy
transmission electron microscopy
microscopy
Water quality

これを引用

Suherman, HARYANTO, NICKO. AUDIO., WAHYUNI, ENDANG. TRI., ILMI, MIFTAHUL., MORITA, KINICHI., & Oki, Y. (2019). Carbon Dots Modification for Escherichia coli Detection: Variation of Colistin Sulphate Concentration. Oriental Journal of Chemistry, 35(1), 49-55. https://doi.org/10.13005/350105

Carbon Dots Modification for Escherichia coli Detection: Variation of Colistin Sulphate Concentration. / Suherman; HARYANTO, NICKO AUDIO; WAHYUNI, ENDANG TRI; ILMI, MIFTAHUL; MORITA, KINICHI; Oki, Yuji.

:: Oriental Journal of Chemistry, 巻 35, 番号 1, 2019, p. 49-55.

研究成果: ジャーナルへの寄稿記事

Suherman, HARYANTO, NICKOAUDIO, WAHYUNI, ENDANGTRI, ILMI, MIFTAHUL, MORITA, KINICHI & Oki, Y 2019, 'Carbon Dots Modification for Escherichia coli Detection: Variation of Colistin Sulphate Concentration', Oriental Journal of Chemistry, 巻. 35, 番号 1, pp. 49-55. https://doi.org/10.13005/350105
Suherman ; HARYANTO, NICKO AUDIO ; WAHYUNI, ENDANG TRI ; ILMI, MIFTAHUL ; MORITA, KINICHI ; Oki, Yuji. / Carbon Dots Modification for Escherichia coli Detection: Variation of Colistin Sulphate Concentration. :: Oriental Journal of Chemistry. 2019 ; 巻 35, 番号 1. pp. 49-55.
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abstract = "Escherichia coli bacteria widely recognize as a major treatment in water quality as diarrhea disease becoming one of leading causes of mortality worldwide. Carbon dots (CDs) modification study for detection of E. coli with the variation of colistin sulphate concentration has been conducted. The purpose of this research is to evaluate the effect of colistin sulphate concentration variation on fluorescence intensity for detecting E. coli bacteria in water samples. This study started by synthesizing CDs and CDs colistin variation concentrations of 1.0; 1.5; 2.0 and 2.5 mmol through carbonization of ammonium citrate and the addition of colistin. All precursors are characterized by using FT-IR. Morphological forms were evaluated with scanning emission microscopy (SEM) and transmission electron microscopy (TEM). CDs and CDs colistin solutions were measured their spectras with a UV-Vis spectrophotometer. Each of these CDs colistin was determined at the excitation wavelength ranges of 310-450 nm using a spectrofluorophotometer. Subsequently, CDs colistin was used for bacterial detection in samples with variation in E. coli bacteria concentration, thus analyzed by spectrofluorophotometer with an optimum excitation wavelength. The results showed that the highest intensity of fluorescence on CDs colistin occurred at the wavelength excitation of 360 nm. CDs colistin with the variation of colistin concentration 2.5 mmol revealed the highest fluorescence intensity. For the detection of E. coli, all CDs colistin produced the same trends of fluorescence spectra as the higher concentration of E. coli in the samples produced the higher intensity of emission.",
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AB - Escherichia coli bacteria widely recognize as a major treatment in water quality as diarrhea disease becoming one of leading causes of mortality worldwide. Carbon dots (CDs) modification study for detection of E. coli with the variation of colistin sulphate concentration has been conducted. The purpose of this research is to evaluate the effect of colistin sulphate concentration variation on fluorescence intensity for detecting E. coli bacteria in water samples. This study started by synthesizing CDs and CDs colistin variation concentrations of 1.0; 1.5; 2.0 and 2.5 mmol through carbonization of ammonium citrate and the addition of colistin. All precursors are characterized by using FT-IR. Morphological forms were evaluated with scanning emission microscopy (SEM) and transmission electron microscopy (TEM). CDs and CDs colistin solutions were measured their spectras with a UV-Vis spectrophotometer. Each of these CDs colistin was determined at the excitation wavelength ranges of 310-450 nm using a spectrofluorophotometer. Subsequently, CDs colistin was used for bacterial detection in samples with variation in E. coli bacteria concentration, thus analyzed by spectrofluorophotometer with an optimum excitation wavelength. The results showed that the highest intensity of fluorescence on CDs colistin occurred at the wavelength excitation of 360 nm. CDs colistin with the variation of colistin concentration 2.5 mmol revealed the highest fluorescence intensity. For the detection of E. coli, all CDs colistin produced the same trends of fluorescence spectra as the higher concentration of E. coli in the samples produced the higher intensity of emission.

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