Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene

Takashi Sakudoh, Hideki Sezutsu, Takeharu Nakashima, Isao Kobayashi, Hirofumi Fujimoto, Keiro Uchino, Yutaka Banno, Hidetoshi Iwano, Hideaki Maekawa, Toshiki Tamura, Hiroshi Kataoka, Kozo Tsuchida

研究成果: ジャーナルへの寄稿記事

83 引用 (Scopus)

抄録

Mechanisms for the uptake and transport of carotenoids, essential nutrients for humans, are not well understood in any animal system. The Y (Yellow blood) gene, a critical cocoon color determinant in the silkworm Bombyx mori, controls the uptake of carotenoids into the intestinal mucosa and the silk gland. Here we provide evidence that the Y gene corresponds to the intracellular carotenoid-binding protein (CBP) gene. In the Y recessive strain, the absence of an exon, likely due to an incorrect mRNA splicing caused by a transposon-associated genomic deletion, generates a nonfunctional CBP mRNA, resulting in colorless hemolymph and white cocoons. Enhancement of carotenoid uptake and coloration of the white cocoon was achieved by germ-line transformation with the CBP gene. This study demonstrates the existence of a genetically facilitated intracellular process beyond passive diffusion for carotenoid uptake in the animal phyla, and paves the way for modulating silk color and lipid content through genetic engineering.

元の言語英語
ページ(範囲)8941-8946
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
104
発行部数21
DOI
出版物ステータス出版済み - 5 22 2007

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Silk
Carotenoids
Carrier Proteins
Genes
Bombyx
Color
Messenger RNA
Genetic Engineering
Hemolymph
Intestinal Mucosa
Germ Cells
Exons
Lipids
Food

All Science Journal Classification (ASJC) codes

  • General

これを引用

Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. / Sakudoh, Takashi; Sezutsu, Hideki; Nakashima, Takeharu; Kobayashi, Isao; Fujimoto, Hirofumi; Uchino, Keiro; Banno, Yutaka; Iwano, Hidetoshi; Maekawa, Hideaki; Tamura, Toshiki; Kataoka, Hiroshi; Tsuchida, Kozo.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 104, 番号 21, 22.05.2007, p. 8941-8946.

研究成果: ジャーナルへの寄稿記事

Sakudoh, T, Sezutsu, H, Nakashima, T, Kobayashi, I, Fujimoto, H, Uchino, K, Banno, Y, Iwano, H, Maekawa, H, Tamura, T, Kataoka, H & Tsuchida, K 2007, 'Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene', Proceedings of the National Academy of Sciences of the United States of America, 巻. 104, 番号 21, pp. 8941-8946. https://doi.org/10.1073/pnas.0702860104
Sakudoh, Takashi ; Sezutsu, Hideki ; Nakashima, Takeharu ; Kobayashi, Isao ; Fujimoto, Hirofumi ; Uchino, Keiro ; Banno, Yutaka ; Iwano, Hidetoshi ; Maekawa, Hideaki ; Tamura, Toshiki ; Kataoka, Hiroshi ; Tsuchida, Kozo. / Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. :: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; 巻 104, 番号 21. pp. 8941-8946.
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