Casein kinase 2 is essential for mitophagy

Tomotake Kanki, Yusuke Kurihara, Xiulian Jin, Tadahiro Goda, Yusuke Ono, Masamune Aihara, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Dongchon Kang

研究成果: ジャーナルへの寄稿記事

65 引用 (Scopus)

抄録

Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

元の言語英語
ページ(範囲)788-794
ページ数7
ジャーナルEMBO Reports
14
発行部数9
DOI
出版物ステータス出版済み - 9 1 2013

Fingerprint

Mitochondrial Degradation
Casein Kinase II
Mitochondria
Yeast
Serine
Phosphorylation
Membrane Proteins
Phosphotransferases
Yeasts
Autophagy
Vacuoles
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

これを引用

Kanki, T., Kurihara, Y., Jin, X., Goda, T., Ono, Y., Aihara, M., ... Kang, D. (2013). Casein kinase 2 is essential for mitophagy. EMBO Reports, 14(9), 788-794. https://doi.org/10.1038/embor.2013.114

Casein kinase 2 is essential for mitophagy. / Kanki, Tomotake; Kurihara, Yusuke; Jin, Xiulian; Goda, Tadahiro; Ono, Yusuke; Aihara, Masamune; Hirota, Yuko; Saigusa, Tetsu; Aoki, Yoshimasa; Uchiumi, Takeshi; Kang, Dongchon.

:: EMBO Reports, 巻 14, 番号 9, 01.09.2013, p. 788-794.

研究成果: ジャーナルへの寄稿記事

Kanki, T, Kurihara, Y, Jin, X, Goda, T, Ono, Y, Aihara, M, Hirota, Y, Saigusa, T, Aoki, Y, Uchiumi, T & Kang, D 2013, 'Casein kinase 2 is essential for mitophagy', EMBO Reports, 巻. 14, 番号 9, pp. 788-794. https://doi.org/10.1038/embor.2013.114
Kanki T, Kurihara Y, Jin X, Goda T, Ono Y, Aihara M その他. Casein kinase 2 is essential for mitophagy. EMBO Reports. 2013 9 1;14(9):788-794. https://doi.org/10.1038/embor.2013.114
Kanki, Tomotake ; Kurihara, Yusuke ; Jin, Xiulian ; Goda, Tadahiro ; Ono, Yusuke ; Aihara, Masamune ; Hirota, Yuko ; Saigusa, Tetsu ; Aoki, Yoshimasa ; Uchiumi, Takeshi ; Kang, Dongchon. / Casein kinase 2 is essential for mitophagy. :: EMBO Reports. 2013 ; 巻 14, 番号 9. pp. 788-794.
@article{6860935f721649fe86df6ef20fad381e,
title = "Casein kinase 2 is essential for mitophagy",
abstract = "Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.",
author = "Tomotake Kanki and Yusuke Kurihara and Xiulian Jin and Tadahiro Goda and Yusuke Ono and Masamune Aihara and Yuko Hirota and Tetsu Saigusa and Yoshimasa Aoki and Takeshi Uchiumi and Dongchon Kang",
year = "2013",
month = "9",
day = "1",
doi = "10.1038/embor.2013.114",
language = "English",
volume = "14",
pages = "788--794",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Casein kinase 2 is essential for mitophagy

AU - Kanki, Tomotake

AU - Kurihara, Yusuke

AU - Jin, Xiulian

AU - Goda, Tadahiro

AU - Ono, Yusuke

AU - Aihara, Masamune

AU - Hirota, Yuko

AU - Saigusa, Tetsu

AU - Aoki, Yoshimasa

AU - Uchiumi, Takeshi

AU - Kang, Dongchon

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

AB - Mitophagy is a process that selectively degrades mitochondria. When mitophagy is induced in yeast, the mitochondrial outer membrane protein Atg32 is phosphorylated, interacts with the adaptor protein Atg11 and is recruited into the vacuole with mitochondria. We screened kinase-deleted yeast strains and found that CK2 is essential for Atg32 phosphorylation, Atg32-Atg11 interaction and mitophagy. Inhibition of CK2 specifically blocks mitophagy, but not macroautophagy, pexophagy or the Cvt pathway. In vitro, CK2 phosphorylates Atg32 at serine 114 and serine 119. We conclude that CK2 regulates mitophagy by directly phosphorylating Atg32.

UR - http://www.scopus.com/inward/record.url?scp=84883487916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883487916&partnerID=8YFLogxK

U2 - 10.1038/embor.2013.114

DO - 10.1038/embor.2013.114

M3 - Article

C2 - 23897086

AN - SCOPUS:84883487916

VL - 14

SP - 788

EP - 794

JO - EMBO Reports

JF - EMBO Reports

SN - 1469-221X

IS - 9

ER -