Catalytic and structural properties of surfactant-horseradish peroxidase complex in organic media

Noriho Kamiya, Masahito Inoue, Masahiro Goto, Nobuhumi Nakamura, Yoshinori Naruta

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

A surfactant-horseradish peroxidase (HRP) complex that is catalytically active in organic media has been successfully prepared by a method utilizing water-in-oil (W/O) emulsions. To optimize conditions for preparation of the HRP complex, the effects of some key parameters in the aqueous phase of W/O emulsions were investigated. The surfactant-HRP complex prepared with a nonionic surfactant exhibited a high catalytic activity compared to those with a cationic or anionic surfactant in anhydrous benzene. At the preparation step, the pH of the aqueous solution had a prominent effect on the enzymatic activity of the HRP complex in organic media. Several kinds of salts present in the HRP complex could be employed to enhance the catalytic performance in organic media. However, anionic ions present in the preparation process appeared to lower the catalytic activity owing to the complexation with heme iron. UV-visible absorption spectra of the HRP complex in benzene, which were prepared from a KCN solution (pH 7.0) or an alkaline solution (pH 12), were comparable with those of native HRP in aqueous solution under the same conditions. Resonance Raman spectroscopic studies also revealed that no significant change in the coordination state of the heme iron occurred even after coating the enzyme with surfactant molecules, lyophilization, and solubilization in nonaqueous media.

元の言語英語
ページ(範囲)52-58
ページ数7
ジャーナルBiotechnology Progress
16
発行部数1
DOI
出版物ステータス出版済み - 1 1 2000

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Horseradish Peroxidase
Surface-Active Agents
Benzene
Emulsions
Heme
Oils
Iron
Freeze Drying
Water
Salts
Ions
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology

これを引用

Catalytic and structural properties of surfactant-horseradish peroxidase complex in organic media. / Kamiya, Noriho; Inoue, Masahito; Goto, Masahiro; Nakamura, Nobuhumi; Naruta, Yoshinori.

:: Biotechnology Progress, 巻 16, 番号 1, 01.01.2000, p. 52-58.

研究成果: ジャーナルへの寄稿記事

Kamiya, Noriho ; Inoue, Masahito ; Goto, Masahiro ; Nakamura, Nobuhumi ; Naruta, Yoshinori. / Catalytic and structural properties of surfactant-horseradish peroxidase complex in organic media. :: Biotechnology Progress. 2000 ; 巻 16, 番号 1. pp. 52-58.
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