リゾチームの触媒機構:無脊椎動物型とニワトリ型の比較から

研究成果: ジャーナルへの寄稿記事

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<p>Based on the structure of invertebrate-type lyzozyme, <i>Venerupis philippinarum</i> lysozyme (<i>Vp</i>L), we designed Asn46Asp/Asp52Ser or Asn46Glu/Asp52Ser hen egg white lysozyme (HEWL) mutants. Both lysozyme mutants possessed lysozyme acitivity like <i>Vp</i>L and HEWL. Although Asn46Glu/Asp52Ser mutant formed a glycosyl adduct in the reaction with the N-acetylglucosamine oligomer, however, the turnover rate of estimated from the glycosyl formation and decomposition rates was only 20% of the observed hydrolysis rate of the substrate. From these results, we discussed the catalytic mechanism of lysozymes.</p>
元の言語Japanese
ページ(範囲)140-143
ページ数4
ジャーナル生物物理
57
発行部数3
DOI
出版物ステータス出版済み - 2017

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