Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response factors to alkaline and salt stress

Akihiro Tominaga, Yujiro Higuchi, Hikari Mori, Makoto Akai, Akiko Suyama, Naotaka Yamada, Kaoru Takegawa

研究成果: ジャーナルへの寄稿記事

抄録

How cells of the fission yeast Schizosaccharomyces pombe respond to alkaline stress is not well understood. Here, to elucidate the molecular mechanism underlying the alkaline stress response in S. pombe, we performed DNA microarray analysis. We found that a homolog of human catechol O-methyltransferase 2 (COMT2) is highly upregulated in S. pombe cells exposed to alkaline conditions. We designated the S. pombe homolog as cmt2+ and also identified its paralog, cmt1+, in the S. pombe genome. Reverse transcription PCR confirmed that both cmt1+ and cmt2+ are upregulated within 1 h of exposure to alkaline stress and downregulated within 30 min of returning to an acidic environment. Moreover, we verified that recombinant Cmt proteins exhibit catechol O-methyltransferase activity. To further characterize the expression of cmt1+ and cmt2+, we carried out an EGFP reporter assay using their promoter sequences, which showed that both genes respond not only to alkaline but also to salt stress. Collectively, our findings indicate that the cmt promoter might be an advantageous expression system for use in S. pombe under alkaline culture conditions.

元の言語英語
ページ(範囲)4881-4887
ページ数7
ジャーナルApplied Microbiology and Biotechnology
103
発行部数12
DOI
出版物ステータス出版済み - 6 18 2019

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Catechol O-Methyltransferase
Schizosaccharomyces
Salts
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Recombinant Proteins
Reverse Transcription
Down-Regulation
Genome
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response factors to alkaline and salt stress. / Tominaga, Akihiro; Higuchi, Yujiro; Mori, Hikari; Akai, Makoto; Suyama, Akiko; Yamada, Naotaka; Takegawa, Kaoru.

:: Applied Microbiology and Biotechnology, 巻 103, 番号 12, 18.06.2019, p. 4881-4887.

研究成果: ジャーナルへの寄稿記事

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abstract = "How cells of the fission yeast Schizosaccharomyces pombe respond to alkaline stress is not well understood. Here, to elucidate the molecular mechanism underlying the alkaline stress response in S. pombe, we performed DNA microarray analysis. We found that a homolog of human catechol O-methyltransferase 2 (COMT2) is highly upregulated in S. pombe cells exposed to alkaline conditions. We designated the S. pombe homolog as cmt2+ and also identified its paralog, cmt1+, in the S. pombe genome. Reverse transcription PCR confirmed that both cmt1+ and cmt2+ are upregulated within 1 h of exposure to alkaline stress and downregulated within 30 min of returning to an acidic environment. Moreover, we verified that recombinant Cmt proteins exhibit catechol O-methyltransferase activity. To further characterize the expression of cmt1+ and cmt2+, we carried out an EGFP reporter assay using their promoter sequences, which showed that both genes respond not only to alkaline but also to salt stress. Collectively, our findings indicate that the cmt promoter might be an advantageous expression system for use in S. pombe under alkaline culture conditions.",
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AU - Akai, Makoto

AU - Suyama, Akiko

AU - Yamada, Naotaka

AU - Takegawa, Kaoru

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