Cationic comb-type copolymer excludes intercalating dye from DNA without inducing DNA condensation

Atsushi Maruyama, Naotaka Sonda, Kohei Yamasaki, Masanori Hirano, Satoru Kidoaki, Naohiko Shimada, Masatoshi Maeshiro, Masaya Miyazaki

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

The interaction of a single molecule of DNA with cationic comb-type copolymers has been observed using a flow-stretch assay. We have shown that interaction with a copolymer with a high degree of grafting did not cause a coil-to-globule transition but resulted in shrinkage of the DNA by 20%. In this study, a flow-stretch assay was employed in which changes in length and fluorescence intensity of DNA were simultaneously observed. Upon binding of fluorescent intercalator dyes, the DNA length was extended. In the presence of copolymer, dye binding was inhibited and DNA length was not significantly different than that in the absence of dye and copolymer. Our data suggest that the copolymer does not influence DNA length. Our observation implies that use of the fluorescence dye exclusion assay to estimate DNA condensing activity of polycations must be carefully carried out.

本文言語英語
ページ(範囲)185-188
ページ数4
ジャーナルCurrent Nanoscience
10
2
DOI
出版ステータス出版済み - 2014

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 医学(その他)
  • 生体医工学
  • 薬科学

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