cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells

Yasuyuki Hayashi, Tohru Kiyono, Masatoshi Fujita, Masahide Ishibashi

研究成果: ジャーナルへの寄稿学術誌査読

21 被引用数 (Scopus)

抄録

A novel cDNA fragment, named cca1 (confluent 3Y1 cell-associated 1), was previously isolated on the basis of preferential accumulation of the corresponding mRNA in growth-arrested confluent but not in growing subconfluent rat 3Y1 cells. The cca1 cDNA was found to consist of 5022 nucleotides with an open reading frame of 1905 nucleotides, encoding a protein of 635 amino acids. Unlike the 3Y1 cell case, cca1 mRNA was not detected in confluent 3Y1 BU, 3Y1 BU/pTK, 3Y1-16E6, or F2408 cells, whose growth patterns monitored by phalloidin staining and bromodeoxyuridine incorporation were different from those of the confluent 3Y1 cells. A restoration of the confluent 3Y1-type growth pattern was observed in the cca1 cDNA-introduced 3Y1 BU and 3Y1 BU/pTK cells after reaching confluence but not in the cDNA-introduced 3Y1-16E6 or F2408 cells. The results allow us to conclude that cca1 is required but not sufficient for formation of growth- arrested confluent monolayer of 3Y1 cells.

本文言語英語
ページ(範囲)18082-18086
ページ数5
ジャーナルJournal of Biological Chemistry
272
29
DOI
出版ステータス出版済み - 7月 18 1997
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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