CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma

Keigo Kubota, Masafumi Moriyama, Sachiko Furukawa, Haque A.S.M. Rafiul, Yasuyuki Maruse, Teppei Jinno, Akihiko Tanaka, Miho Ohta, Noriko Ishiguro, Masaaki Yamauchi, Mizuki Sakamoto, takashi maehara, Hayashida Jun-Nosuke, Shintarou Kawano, Tamotsu Kiyoshima, Seiji Nakamura

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

Tumor-associated macrophages (TAMs) promote cancer cell proliferation, invasion, and metastasis by producing various mediators.Although preclinical studies demonstrated that TAMs preferentially express CD163 and CD204, the TAM subsets in oral squamous cell carcinoma (OSCC) remain unknown.In this study, we examined the expression and role of TAM subsets in OSCC.Forty-six patients with OSCC were analyzed for expression of TAMs in biopsy samples by immunohistochemistry.We examined TAM subsets and their production of immune suppressive molecules (IL-10 and PD-L1) in peripheral blood mononuclear cells from three OSCC patients by flow cytometry.CD163 was detected around the tumor or connective tissue, while CD204 was detected in/around the tumors.Flow cytometric analysis revealed that CD163 + CD204 + TAMs strongly produced IL-10 and PD-L1 in comparison with CD163 + CD204 - and CD163 - CD204 + TAMs.Furthermore, the number of activated CD3 + T cells after co-culture with CD163 + CD204 + TAMs was significantly lower than that after co-culture with other TAM subsets.In clinical findings, the number of CD163 + CD204 + TAMs was negatively correlated with that of CD25 + cells and 5-year progression-free survival.These results suggest that CD163 + CD204 + TAMs possibly play a key role in the invasion and metastasis of OSCC by T-cell regulation via IL-10 and PD-L1 production.

元の言語英語
記事番号1755
ジャーナルScientific reports
7
発行部数1
DOI
出版物ステータス出版済み - 12 1 2017

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Interleukin-10
Squamous Cell Carcinoma
Macrophages
T-Lymphocytes
Neoplasms
Coculture Techniques
Neoplasm Metastasis
Connective Tissue
Disease-Free Survival
Blood Cells
Flow Cytometry
Immunohistochemistry
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • General

これを引用

CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma . / Kubota, Keigo; Moriyama, Masafumi; Furukawa, Sachiko; Rafiul, Haque A.S.M.; Maruse, Yasuyuki; Jinno, Teppei; Tanaka, Akihiko; Ohta, Miho; Ishiguro, Noriko; Yamauchi, Masaaki; Sakamoto, Mizuki; maehara, takashi; Jun-Nosuke, Hayashida; Kawano, Shintarou; Kiyoshima, Tamotsu; Nakamura, Seiji.

:: Scientific reports, 巻 7, 番号 1, 1755, 01.12.2017.

研究成果: ジャーナルへの寄稿記事

Kubota, Keigo ; Moriyama, Masafumi ; Furukawa, Sachiko ; Rafiul, Haque A.S.M. ; Maruse, Yasuyuki ; Jinno, Teppei ; Tanaka, Akihiko ; Ohta, Miho ; Ishiguro, Noriko ; Yamauchi, Masaaki ; Sakamoto, Mizuki ; maehara, takashi ; Jun-Nosuke, Hayashida ; Kawano, Shintarou ; Kiyoshima, Tamotsu ; Nakamura, Seiji. / CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma :: Scientific reports. 2017 ; 巻 7, 番号 1.
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abstract = "Tumor-associated macrophages (TAMs) promote cancer cell proliferation, invasion, and metastasis by producing various mediators.Although preclinical studies demonstrated that TAMs preferentially express CD163 and CD204, the TAM subsets in oral squamous cell carcinoma (OSCC) remain unknown.In this study, we examined the expression and role of TAM subsets in OSCC.Forty-six patients with OSCC were analyzed for expression of TAMs in biopsy samples by immunohistochemistry.We examined TAM subsets and their production of immune suppressive molecules (IL-10 and PD-L1) in peripheral blood mononuclear cells from three OSCC patients by flow cytometry.CD163 was detected around the tumor or connective tissue, while CD204 was detected in/around the tumors.Flow cytometric analysis revealed that CD163 + CD204 + TAMs strongly produced IL-10 and PD-L1 in comparison with CD163 + CD204 - and CD163 - CD204 + TAMs.Furthermore, the number of activated CD3 + T cells after co-culture with CD163 + CD204 + TAMs was significantly lower than that after co-culture with other TAM subsets.In clinical findings, the number of CD163 + CD204 + TAMs was negatively correlated with that of CD25 + cells and 5-year progression-free survival.These results suggest that CD163 + CD204 + TAMs possibly play a key role in the invasion and metastasis of OSCC by T-cell regulation via IL-10 and PD-L1 production.",
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T1 - CD163 + CD204 + tumor-associated macrophages contribute to T cell regulation via interleukin-10 and PD-L1 production in oral squamous cell carcinoma

AU - Kubota, Keigo

AU - Moriyama, Masafumi

AU - Furukawa, Sachiko

AU - Rafiul, Haque A.S.M.

AU - Maruse, Yasuyuki

AU - Jinno, Teppei

AU - Tanaka, Akihiko

AU - Ohta, Miho

AU - Ishiguro, Noriko

AU - Yamauchi, Masaaki

AU - Sakamoto, Mizuki

AU - maehara, takashi

AU - Jun-Nosuke, Hayashida

AU - Kawano, Shintarou

AU - Kiyoshima, Tamotsu

AU - Nakamura, Seiji

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Tumor-associated macrophages (TAMs) promote cancer cell proliferation, invasion, and metastasis by producing various mediators.Although preclinical studies demonstrated that TAMs preferentially express CD163 and CD204, the TAM subsets in oral squamous cell carcinoma (OSCC) remain unknown.In this study, we examined the expression and role of TAM subsets in OSCC.Forty-six patients with OSCC were analyzed for expression of TAMs in biopsy samples by immunohistochemistry.We examined TAM subsets and their production of immune suppressive molecules (IL-10 and PD-L1) in peripheral blood mononuclear cells from three OSCC patients by flow cytometry.CD163 was detected around the tumor or connective tissue, while CD204 was detected in/around the tumors.Flow cytometric analysis revealed that CD163 + CD204 + TAMs strongly produced IL-10 and PD-L1 in comparison with CD163 + CD204 - and CD163 - CD204 + TAMs.Furthermore, the number of activated CD3 + T cells after co-culture with CD163 + CD204 + TAMs was significantly lower than that after co-culture with other TAM subsets.In clinical findings, the number of CD163 + CD204 + TAMs was negatively correlated with that of CD25 + cells and 5-year progression-free survival.These results suggest that CD163 + CD204 + TAMs possibly play a key role in the invasion and metastasis of OSCC by T-cell regulation via IL-10 and PD-L1 production.

AB - Tumor-associated macrophages (TAMs) promote cancer cell proliferation, invasion, and metastasis by producing various mediators.Although preclinical studies demonstrated that TAMs preferentially express CD163 and CD204, the TAM subsets in oral squamous cell carcinoma (OSCC) remain unknown.In this study, we examined the expression and role of TAM subsets in OSCC.Forty-six patients with OSCC were analyzed for expression of TAMs in biopsy samples by immunohistochemistry.We examined TAM subsets and their production of immune suppressive molecules (IL-10 and PD-L1) in peripheral blood mononuclear cells from three OSCC patients by flow cytometry.CD163 was detected around the tumor or connective tissue, while CD204 was detected in/around the tumors.Flow cytometric analysis revealed that CD163 + CD204 + TAMs strongly produced IL-10 and PD-L1 in comparison with CD163 + CD204 - and CD163 - CD204 + TAMs.Furthermore, the number of activated CD3 + T cells after co-culture with CD163 + CD204 + TAMs was significantly lower than that after co-culture with other TAM subsets.In clinical findings, the number of CD163 + CD204 + TAMs was negatively correlated with that of CD25 + cells and 5-year progression-free survival.These results suggest that CD163 + CD204 + TAMs possibly play a key role in the invasion and metastasis of OSCC by T-cell regulation via IL-10 and PD-L1 production.

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