CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

A. S.M.Rafiul Haque, Masafumi Moriyama, Keigo Kubota, Noriko Ishiguro, Mizuki Sakamoto, Akira Chinju, Keita Mochizuki, Taiki Sakamoto, Naoki Kaneko, Ryusuke Munemura, Takashi Maehara, Akihiko Tanaka, Jun Nosuke Hayashida, Shintaro Kawano, Tamotsu Kiyoshima, Seiji Nakamura

研究成果: ジャーナルへの寄稿記事

抄録

Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators and preferentially express CD163, CD204, and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Here we investigated the expression and function of TAM subsets in OSCC, especially in cancer cell proliferation. Biopsy sample from 44 patients with OSCC were examined for the expression of TAM markers and EGF by immunohistochemistry. EGF production of TAM subsets isolated from OSCC patients was assessed by flow cytometry. We also examined the effect of conditioned medium from TAM subsets on the proliferation of OSCC cells. CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis found that CD206+ TAMs strongly produced EGF compared with CD163+ and CD204+ TAMs. Cell proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with worse clinical prognosis. Our results revealed differences in localization and EGF production among these TAM subsets. CD206+ TAMs might play a critical role in the proliferation of OSCC via EGF production.

元の言語英語
記事番号14611
ジャーナルScientific reports
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2019

Fingerprint

Epidermal Growth Factor
Squamous Cell Carcinoma
Macrophages
Neoplasms
Conditioned Culture Medium
Cell Proliferation
Connective Tissue
Cultured Cells

All Science Journal Classification (ASJC) codes

  • General

これを引用

CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production. / Haque, A. S.M.Rafiul; Moriyama, Masafumi; Kubota, Keigo; Ishiguro, Noriko; Sakamoto, Mizuki; Chinju, Akira; Mochizuki, Keita; Sakamoto, Taiki; Kaneko, Naoki; Munemura, Ryusuke; Maehara, Takashi; Tanaka, Akihiko; Hayashida, Jun Nosuke; Kawano, Shintaro; Kiyoshima, Tamotsu; Nakamura, Seiji.

:: Scientific reports, 巻 9, 番号 1, 14611, 01.12.2019.

研究成果: ジャーナルへの寄稿記事

Haque, ASMR, Moriyama, M, Kubota, K, Ishiguro, N, Sakamoto, M, Chinju, A, Mochizuki, K, Sakamoto, T, Kaneko, N, Munemura, R, Maehara, T, Tanaka, A, Hayashida, JN, Kawano, S, Kiyoshima, T & Nakamura, S 2019, 'CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production', Scientific reports, 巻. 9, 番号 1, 14611. https://doi.org/10.1038/s41598-019-51149-1
Haque, A. S.M.Rafiul ; Moriyama, Masafumi ; Kubota, Keigo ; Ishiguro, Noriko ; Sakamoto, Mizuki ; Chinju, Akira ; Mochizuki, Keita ; Sakamoto, Taiki ; Kaneko, Naoki ; Munemura, Ryusuke ; Maehara, Takashi ; Tanaka, Akihiko ; Hayashida, Jun Nosuke ; Kawano, Shintaro ; Kiyoshima, Tamotsu ; Nakamura, Seiji. / CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production. :: Scientific reports. 2019 ; 巻 9, 番号 1.
@article{e5aa1969f40b4453905be9e44107b6ea,
title = "CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production",
abstract = "Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators and preferentially express CD163, CD204, and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Here we investigated the expression and function of TAM subsets in OSCC, especially in cancer cell proliferation. Biopsy sample from 44 patients with OSCC were examined for the expression of TAM markers and EGF by immunohistochemistry. EGF production of TAM subsets isolated from OSCC patients was assessed by flow cytometry. We also examined the effect of conditioned medium from TAM subsets on the proliferation of OSCC cells. CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis found that CD206+ TAMs strongly produced EGF compared with CD163+ and CD204+ TAMs. Cell proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with worse clinical prognosis. Our results revealed differences in localization and EGF production among these TAM subsets. CD206+ TAMs might play a critical role in the proliferation of OSCC via EGF production.",
author = "Haque, {A. S.M.Rafiul} and Masafumi Moriyama and Keigo Kubota and Noriko Ishiguro and Mizuki Sakamoto and Akira Chinju and Keita Mochizuki and Taiki Sakamoto and Naoki Kaneko and Ryusuke Munemura and Takashi Maehara and Akihiko Tanaka and Hayashida, {Jun Nosuke} and Shintaro Kawano and Tamotsu Kiyoshima and Seiji Nakamura",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-51149-1",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

AU - Haque, A. S.M.Rafiul

AU - Moriyama, Masafumi

AU - Kubota, Keigo

AU - Ishiguro, Noriko

AU - Sakamoto, Mizuki

AU - Chinju, Akira

AU - Mochizuki, Keita

AU - Sakamoto, Taiki

AU - Kaneko, Naoki

AU - Munemura, Ryusuke

AU - Maehara, Takashi

AU - Tanaka, Akihiko

AU - Hayashida, Jun Nosuke

AU - Kawano, Shintaro

AU - Kiyoshima, Tamotsu

AU - Nakamura, Seiji

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators and preferentially express CD163, CD204, and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Here we investigated the expression and function of TAM subsets in OSCC, especially in cancer cell proliferation. Biopsy sample from 44 patients with OSCC were examined for the expression of TAM markers and EGF by immunohistochemistry. EGF production of TAM subsets isolated from OSCC patients was assessed by flow cytometry. We also examined the effect of conditioned medium from TAM subsets on the proliferation of OSCC cells. CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis found that CD206+ TAMs strongly produced EGF compared with CD163+ and CD204+ TAMs. Cell proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with worse clinical prognosis. Our results revealed differences in localization and EGF production among these TAM subsets. CD206+ TAMs might play a critical role in the proliferation of OSCC via EGF production.

AB - Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators and preferentially express CD163, CD204, and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Here we investigated the expression and function of TAM subsets in OSCC, especially in cancer cell proliferation. Biopsy sample from 44 patients with OSCC were examined for the expression of TAM markers and EGF by immunohistochemistry. EGF production of TAM subsets isolated from OSCC patients was assessed by flow cytometry. We also examined the effect of conditioned medium from TAM subsets on the proliferation of OSCC cells. CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis found that CD206+ TAMs strongly produced EGF compared with CD163+ and CD204+ TAMs. Cell proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with worse clinical prognosis. Our results revealed differences in localization and EGF production among these TAM subsets. CD206+ TAMs might play a critical role in the proliferation of OSCC via EGF production.

UR - http://www.scopus.com/inward/record.url?scp=85073118218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073118218&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-51149-1

DO - 10.1038/s41598-019-51149-1

M3 - Article

C2 - 31601953

AN - SCOPUS:85073118218

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14611

ER -