Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy

Jingchuan Sun, Hironori Kawakami, Juergen Zech, Christian Speck, Bruce Stillman, Huilin Li

研究成果: ジャーナルへの寄稿記事

47 引用 (Scopus)

抄録

The eukaryotic origin recognition complex (ORC) interacts with and remodels origins of DNA replication prior to initiation in S phase. Here, we report a single-particle cryo-EM-derived structure of the supramolecular assembly comprising Saccharomyces cerevisiae ORC, the replication initiation factor Cdc6, and double-stranded ARS1 origin DNA in the presence of ATPγS. The six subunits of ORC are arranged as Orc1:Orc4:Orc5:Orc2:Orc3, with Orc6 binding to Orc2. Cdc6 binding changes the conformation of ORC, in particular reorienting the Orc1 N-terminal BAH domain. Segmentation of the 3D map of ORC-Cdc6 on DNA and docking with the crystal structure of the homologous archaeal Orc1/Cdc6 protein suggest an origin DNA binding model in which the DNA tracks along the interior surface of the crescent-like ORC. Thus, ORC bends and wraps the DNA. This model is consistent with the observation that binding of a single Cdc6 extends the ORC footprint on origin DNA from both ends.

元の言語英語
ページ(範囲)534-544
ページ数11
ジャーナルStructure
20
発行部数3
DOI
出版物ステータス出版済み - 3 7 2012

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Origin Recognition Complex
Cryoelectron Microscopy
DNA
Peptide Initiation Factors
Replication Origin
DNA Replication
S Phase
Saccharomyces cerevisiae

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

これを引用

Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy. / Sun, Jingchuan; Kawakami, Hironori; Zech, Juergen; Speck, Christian; Stillman, Bruce; Li, Huilin.

:: Structure, 巻 20, 番号 3, 07.03.2012, p. 534-544.

研究成果: ジャーナルへの寄稿記事

Sun, J, Kawakami, H, Zech, J, Speck, C, Stillman, B & Li, H 2012, 'Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy', Structure, 巻. 20, 番号 3, pp. 534-544. https://doi.org/10.1016/j.str.2012.01.011
Sun, Jingchuan ; Kawakami, Hironori ; Zech, Juergen ; Speck, Christian ; Stillman, Bruce ; Li, Huilin. / Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy. :: Structure. 2012 ; 巻 20, 番号 3. pp. 534-544.
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