CDK phosphorylation regulates Mcm3 degradation in budding yeast

Kaori Yamamoto, Nishiho Makino, Masayoshi Nagai, Hiroyuki Araki, Takashi Ushimaru

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

Accurate regulation of activity and level of the MCM complex is critical for precise DNA replication and genome transmission. Cyclin-dependent kinase (CDK) negatively regulates nuclear localization of the MCM complex via phosphorylation of the Mcm3 subunit. More recently, we found that Mcm3 is degraded via the Skp1–Cullin–F-box (SCF)–proteasome axis in budding yeast. However, how Mcm3 degradation is regulated is largely unknown. Here, we show that CDK represses Mcm3 degradation. Phosphorylated Mcm3 was excluded from the nucleus, where SCF is predominantly located, although CDK-mediated phosphorylation itself generated a phosphodegron of Mcm3, stimulating the degradation of Mcm3 resident in the nucleus. Thus, CDK negatively regulated nuclear MCM levels by exclusion from the nucleus and degradation in the nucleus via Mcm3 phosphorylation. We will discuss the physiological importance of Mcm3 degradation.

本文言語英語
ページ(範囲)680-684
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
506
3
DOI
出版ステータス出版済み - 11月 30 2018
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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