CDNA cloning and characterization of the third isoform of human peroxin Pex11p

Atsushi Tanaka, Kanji Okumoto, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

33 引用 (Scopus)

抄録

We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.

元の言語英語
ページ(範囲)819-823
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
300
発行部数4
DOI
出版物ステータス出版済み - 1 24 2003

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Cloning
Organism Cloning
Protein Isoforms
Peroxisome Proliferators
Chromosomes, Human, Pair 19
Amino Acids
Clofibrate
Protein Databases
Peroxisomes
Transcription
Fibroblasts
Chromosomes
Cricetulus
Cytosol
Reverse Transcription
Rats
Epitopes
Ovary
Membrane Proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

CDNA cloning and characterization of the third isoform of human peroxin Pex11p. / Tanaka, Atsushi; Okumoto, Kanji; Fujiki, Yukio.

:: Biochemical and Biophysical Research Communications, 巻 300, 番号 4, 24.01.2003, p. 819-823.

研究成果: ジャーナルへの寄稿記事

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abstract = "We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22{\%} and 23{\%} amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.",
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N2 - We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.

AB - We have cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pγ, by BLASTP homology search on eukaryotic protein database and reverse transcription (RT)-PCR of human fibroblast RNA. Pex11pγ was 241 amino-acid long, with two putative transmembrane segments, showing 22% and 23% amino-acid identity to Pex11pα and Pex11pβ, respectively. PEX11γ gene was located on chromosome 19, distinct from PEX11α and PEX11β. Pex11pγ was found to be a peroxisomal membrane protein, as assessed by colocalization with peroxisome targeting signal type 1 (PTS1)-proteins, in epitope-tagged Pex11pγ-expressing Chinese hamster ovary cells. Pex11pγ exposes both of the N- and C-terminal parts to the cytosol. PEX11γ was not induced in rats by treatment of clofibrate, a peroxisome proliferator, similar to constitutively expressed PEX11β but in contrast to inducible PEX11α.

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