C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis

Sakiko Satake, Hideyo Hirai, Yoshihiro Hayashi, Nobuaki Shime, Akihiro Tamura, Hisayuki Yao, Satoshi Yoshioka, Yasuo Miura, Tohru Inaba, Naohisa Fujita, Eishi Ashihara, Jiro Imanishi, Teiji Sawa, Taira Maekawa

研究成果: Contribution to journalArticle査読

53 被引用数 (Scopus)

抄録

Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPβ is required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations (#1-5) according to their levels of c-Kit and Ly-6G expression. After the induction of candidemia, rapid mobilization of mature granulocytes and an increase in early granulocyte precursors accompanied by cell cycle acceleration was followed by a gradual increase in granulocytes originating from the immature populations. Upon infection, C/EBPβ was upregulated at the protein level in all the granulopoietic subpopulations. The rapid increase in immature subpopulations #1 and #2 observed in C/EBPβ knockout mice at 1 d postinfection was attenuated. Candidemia-induced cell cycle acceleration and proliferation of hematopoietic stem/progenitors were also impaired. Taken together, these data suggest that C/EBPβ is involved in the efficient amplification of early granulocyte precursors during candidemia-induced emergency granulopoiesis.

本文言語英語
ページ(範囲)4546-4555
ページ数10
ジャーナルJournal of Immunology
189
9
DOI
出版ステータス出版済み - 11 1 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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