Cell cycle-dependent expression of mammalian E2-C regulated by the anaphase-promoting complex/cyclosome

A. Yamanaka, S. Hatakeyama, K. I. Kominami, M. Kitagawa, M. Matsumoto, K. I. Nakayama

研究成果: ジャーナルへの寄稿記事

42 引用 (Scopus)

抄録

Progression through mitosis requires the precisely timed ubiquitin-dependent degradation of specific substrates. E2-C is a ubiquitin-conjugating enzyme that plays a critical role with anaphase-promoting complex/cyclosome (APC/C) in progression of and exit from M phase. Here we report that mammalian E2-C is expressed in late G2/M phase and is degraded as cells exit from M phase. The mammalian E2-C shows an autoubiquitinating activity leading to covalent conjugation to itself with several ubiquitins. The ubiquitination of E2-C is strongly enhanced by APC/C, resulting in the formation of a polyubiquitin chain. The polyubiquitination of mammalian E2-C occurs only when cells exit from M phase. Furthermore, mammalian E2-C contains two putative destruction boxes that are believed to act as recognition motifs for APC/C. The mutation of this motif reduced the polyubiquitination of mammalian E2-C, resulting in its stabilization. These results suggest that mammalian E2-C is itself a substrate of the APC/C-dependent proteolysis machinery, and that the periodic expression of mammalian E2-C may be a novel autoregulatory system for the control of the APC/C activity and its substrate specificity.

元の言語英語
ページ(範囲)2821-2831
ページ数11
ジャーナルMolecular biology of the cell
11
発行部数8
DOI
出版物ステータス出版済み - 1 1 2000

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Anaphase-Promoting Complex-Cyclosome
Cell Division
Cell Cycle
Ubiquitins
Polyubiquitin
Ubiquitin-Conjugating Enzymes
G2 Phase
Ubiquitination
Ubiquitin
Substrate Specificity
Mitosis
Proteolysis
Mutation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

Cell cycle-dependent expression of mammalian E2-C regulated by the anaphase-promoting complex/cyclosome. / Yamanaka, A.; Hatakeyama, S.; Kominami, K. I.; Kitagawa, M.; Matsumoto, M.; Nakayama, K. I.

:: Molecular biology of the cell, 巻 11, 番号 8, 01.01.2000, p. 2821-2831.

研究成果: ジャーナルへの寄稿記事

Yamanaka, A. ; Hatakeyama, S. ; Kominami, K. I. ; Kitagawa, M. ; Matsumoto, M. ; Nakayama, K. I. / Cell cycle-dependent expression of mammalian E2-C regulated by the anaphase-promoting complex/cyclosome. :: Molecular biology of the cell. 2000 ; 巻 11, 番号 8. pp. 2821-2831.
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