Cell fusion through a microslit between adhered cells and observation of their nuclear behavior

Ken Ichi Wada, Kazuo Hosokawa, Eitaro Kondo, Yoshihiro Ito, Mizuo Maeda

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

This paper describes a novel cell fusion method which induces cell fusion between adhered cells through a microslit for preventing nuclear mixing. For this purpose, a microfluidic device which had ∼100 cell pairing structures (CPSs) making cell pairs through microslits with 2.1±0.3μm width was fabricated. After trapping NIH3T3 cells with hydrodynamic forces at the CPSs, the cells were fused through the microslit by the Sendai virus envelope method. With following timelapse observation, we discovered that the spread cells were much less susceptible to nuclear migration passing through the microslit compared with round cells, and that cytoplasmic fraction containing mitochondria was transferred through the microslit without nuclear mixing. These findings will provide an effective method for cell fusion without nuclear mixing, and will lead to an efficient method for reprograming and transdifferentiation of target cells toward regenerative medicine.

本文言語英語
ページ(範囲)1464-1468
ページ数5
ジャーナルBiotechnology and Bioengineering
111
7
DOI
出版ステータス出版済み - 7 2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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