Cell growth and nutrient uptake by cell suspensions of Cupressus lusitanica

Junko Yamada, Koki Fujita, Kazumi Eto, Kokki Sakai

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Conditions for cell growth of suspension cultures of Cupressus lusitanica, which has high β-thujaplicin productivity, were studied. The medium that provided the highest growth rate was IS-1 medium (pH 5.5), modified from Gamborg B5 medium containing 32mM of total nitrogen. Its NO3-N/ NH4-N ratio was 30:2. The maximum growth represented a 25-fold increase over the initial biomass on a fresh weight basis after 30 days of culture in this medium. The highest cell growth was obtained with an initial pH of 3.5-5.5, but the pH of the medium settled to about pH 4.0 from any of the initial pH values in this report. The cells cultured under this condition were able to produce a high level of β-thujaplicin.

本文言語英語
ページ(範囲)5-10
ページ数6
ジャーナルJournal of Wood Science
49
1
DOI
出版ステータス出版済み - 10 31 2003

All Science Journal Classification (ASJC) codes

  • 生体材料

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