Cell mechanical characterization based on on-chip robotics

Fumihito Arai, Shinya Sakuma

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

In this chapter, we propose “on-chip robotics” that is based on robot technologies and lab-on-a-chip devices to achieve high-speed, high-accuracy, and quantitative cell manipulation and analysis on a chip. We presents a high-throughput cell mechanical characterization method using a robot integrated microfluidic chip (robochip). The robochip contains a magnetically driven on-chip probe, a force sensor, and microchannels. The displacement reduction mechanism is adopted to the on-chip probe for high resolution positioning of the tip of the probe, and we achieved noncontact actuation of the on-chip probe with 0.18 μm in repetitive positioning. We show the automated mechanical characterization of oocyte. The throughput of the measurement is approximately 15–20 s per oocyte, and we conclude that high-throughput cellular mechanical characterization is achieved. We also show measurements of the viscoelastic properties of oocyte using the robochip. The proposed approach based on a robochip is a promising technology for contributing to the analysis of the physical and biological properties of cells.

本文言語英語
ホスト出版物のタイトルHyper Bio Assembler for 3D Cellular Systems
出版社Springer Japan
ページ3-22
ページ数20
ISBN(電子版)9784431552970
ISBN(印刷版)9784431552963
DOI
出版ステータス出版済み - 1 1 2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 工学(全般)
  • 医学(全般)
  • 免疫学および微生物学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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