Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement

Yuji Oshikawa, Kazuyuki Furuta, Satoshi Tanaka, Akio Ojida

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.

元の言語英語
ページ(範囲)1526-1529
ページ数4
ジャーナルAnalytical chemistry
88
発行部数3
DOI
出版物ステータス出版済み - 2 2 2016

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Fluorescent Dyes
Histamine
Imaging techniques
Fluorescence
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement. / Oshikawa, Yuji; Furuta, Kazuyuki; Tanaka, Satoshi; Ojida, Akio.

:: Analytical chemistry, 巻 88, 番号 3, 02.02.2016, p. 1526-1529.

研究成果: ジャーナルへの寄稿記事

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abstract = "Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.",
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AU - Tanaka, Satoshi

AU - Ojida, Akio

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AB - Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.

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