Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation

Masaki Tsuchiya, Yuji Hara, Masaki Okuda, Karin Itoh, Ryotaro Nishioka, Akifumi Shiomi, Kohjiro Nagao, Masayuki Mori, Yasuo Mori, Junichi Ikenouchi, Ryo Suzuki, Motomu Tanaka, Tomohiko Ohwada, Junken Aoki, Motoi Kanagawa, Tatsushi Toda, Yosuke Nagata, Ryoichi Matsuda, Yasunori Takayama, Makoto TominagaMasato Umeda

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Myotube formation by fusion of myoblasts and subsequent elongation of the syncytia is essential for skeletal muscle formation. However, molecules that regulate myotube formation remain elusive. Here we identify PIEZO1, a mechanosensitive Ca2+ channel, as a key regulator of myotube formation. During myotube formation, phosphatidylserine, a phospholipid that resides in the inner leaflet of the plasma membrane, is transiently exposed to cell surface and promotes myoblast fusion. We show that cell surface phosphatidylserine inhibits PIEZO1 and that the inward translocation of phosphatidylserine, which is driven by the phospholipid flippase complex of ATP11A and CDC50A, is required for PIEZO1 activation. PIEZO1-mediated Ca2+ influx promotes RhoA/ROCK-mediated actomyosin assemblies at the lateral cortex of myotubes, thus preventing uncontrolled fusion of myotubes and leading to polarized elongation during myotube formation. These results suggest that cell surface flip-flop of phosphatidylserine acts as a molecular switch for PIEZO1 activation that governs proper morphogenesis during myotube formation.

元の言語英語
記事番号2049
ジャーナルNature communications
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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flip-flops
Flip flop circuits
Phosphatidylserines
Skeletal Muscle Fibers
Fusion reactions
cells
Elongation
Phospholipids
Chemical activation
fusion
Actomyosin
Myoblasts
Cell membranes
elongation
Muscle
activation
Switches
skeletal muscle
cortexes
regulators

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Tsuchiya, M., Hara, Y., Okuda, M., Itoh, K., Nishioka, R., Shiomi, A., ... Umeda, M. (2018). Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nature communications, 9(1), [2049]. https://doi.org/10.1038/s41467-018-04436-w

Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. / Tsuchiya, Masaki; Hara, Yuji; Okuda, Masaki; Itoh, Karin; Nishioka, Ryotaro; Shiomi, Akifumi; Nagao, Kohjiro; Mori, Masayuki; Mori, Yasuo; Ikenouchi, Junichi; Suzuki, Ryo; Tanaka, Motomu; Ohwada, Tomohiko; Aoki, Junken; Kanagawa, Motoi; Toda, Tatsushi; Nagata, Yosuke; Matsuda, Ryoichi; Takayama, Yasunori; Tominaga, Makoto; Umeda, Masato.

:: Nature communications, 巻 9, 番号 1, 2049, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Tsuchiya, M, Hara, Y, Okuda, M, Itoh, K, Nishioka, R, Shiomi, A, Nagao, K, Mori, M, Mori, Y, Ikenouchi, J, Suzuki, R, Tanaka, M, Ohwada, T, Aoki, J, Kanagawa, M, Toda, T, Nagata, Y, Matsuda, R, Takayama, Y, Tominaga, M & Umeda, M 2018, 'Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation', Nature communications, 巻. 9, 番号 1, 2049. https://doi.org/10.1038/s41467-018-04436-w
Tsuchiya, Masaki ; Hara, Yuji ; Okuda, Masaki ; Itoh, Karin ; Nishioka, Ryotaro ; Shiomi, Akifumi ; Nagao, Kohjiro ; Mori, Masayuki ; Mori, Yasuo ; Ikenouchi, Junichi ; Suzuki, Ryo ; Tanaka, Motomu ; Ohwada, Tomohiko ; Aoki, Junken ; Kanagawa, Motoi ; Toda, Tatsushi ; Nagata, Yosuke ; Matsuda, Ryoichi ; Takayama, Yasunori ; Tominaga, Makoto ; Umeda, Masato. / Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. :: Nature communications. 2018 ; 巻 9, 番号 1.
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