Cellulase productivity of Trichoderma reesei mutants developed in Japan varies with varying pH conditions

Hiroki Hirasawa, Koki Shioya, Kazuki Mori, Kosuke Tashiro, Sachiyo Aburatani, Yosuke Shida, Satoru Kuhara, Wataru Ogasawara

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The ascomycete Trichoderma reesei is known to produce a variety of cellulases and hemicellulases and the hyper-cellulolytic mutants of this fungus are useful as industrial cellulase producers. In Japan, PC-3-7, derived from the early mutant QM9414, is well-known as a cellulase hyperproducing mutant. In addition to the productivity of enzymes, the composition of secreted enzymes greatly influences biomass saccharification. Therefore, we evaluated the cellulase productivity of T. reesei mutants in Japan at different pH as a factor influencing enzyme production. At higher pH values, QM9414 exhibited reduced cellulase productivity whereas PC-3-7 maintained high cellulase productivity and gene expression at the transcriptional level. The gene encoding the pH-responsive transcription factor PACI did not mutate in PC-3-7, and its expression pattern against different pH conditions was similar between QM9414 and PC-3-7. Furthermore, the deletion of pac1 encoding PACI caused different expression patterns of cellulase genes between QM9414 and PC-3-7. Therefore, we suggest that T. reesei possesses a pH-responsive cellulase production mechanism that is different from the PACI-related mechanism. Finally, we identified that N-25, a strain developed at an early stage of mutant development acquired cellulase productivity at a higher pH. In this investigation, we also found and tested candidate genes possibly affecting pH response using comparative genome analysis.

元の言語英語
ページ(範囲)264-273
ページ数10
ジャーナルJournal of Bioscience and Bioengineering
128
発行部数3
DOI
出版物ステータス出版済み - 9 2019

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Trichoderma
Cellulase
Japan
Productivity
Enzymes
Genes
Saccharification
Gene encoding
Transcription factors
Fungi
Gene expression
Biomass
Cellulases
Ascomycota
Chemical analysis
Transcription Factors
Genome
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Cellulase productivity of Trichoderma reesei mutants developed in Japan varies with varying pH conditions. / Hirasawa, Hiroki; Shioya, Koki; Mori, Kazuki; Tashiro, Kosuke; Aburatani, Sachiyo; Shida, Yosuke; Kuhara, Satoru; Ogasawara, Wataru.

:: Journal of Bioscience and Bioengineering, 巻 128, 番号 3, 09.2019, p. 264-273.

研究成果: ジャーナルへの寄稿記事

Hirasawa, Hiroki ; Shioya, Koki ; Mori, Kazuki ; Tashiro, Kosuke ; Aburatani, Sachiyo ; Shida, Yosuke ; Kuhara, Satoru ; Ogasawara, Wataru. / Cellulase productivity of Trichoderma reesei mutants developed in Japan varies with varying pH conditions. :: Journal of Bioscience and Bioengineering. 2019 ; 巻 128, 番号 3. pp. 264-273.
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abstract = "The ascomycete Trichoderma reesei is known to produce a variety of cellulases and hemicellulases and the hyper-cellulolytic mutants of this fungus are useful as industrial cellulase producers. In Japan, PC-3-7, derived from the early mutant QM9414, is well-known as a cellulase hyperproducing mutant. In addition to the productivity of enzymes, the composition of secreted enzymes greatly influences biomass saccharification. Therefore, we evaluated the cellulase productivity of T. reesei mutants in Japan at different pH as a factor influencing enzyme production. At higher pH values, QM9414 exhibited reduced cellulase productivity whereas PC-3-7 maintained high cellulase productivity and gene expression at the transcriptional level. The gene encoding the pH-responsive transcription factor PACI did not mutate in PC-3-7, and its expression pattern against different pH conditions was similar between QM9414 and PC-3-7. Furthermore, the deletion of pac1 encoding PACI caused different expression patterns of cellulase genes between QM9414 and PC-3-7. Therefore, we suggest that T. reesei possesses a pH-responsive cellulase production mechanism that is different from the PACI-related mechanism. Finally, we identified that N-25, a strain developed at an early stage of mutant development acquired cellulase productivity at a higher pH. In this investigation, we also found and tested candidate genes possibly affecting pH response using comparative genome analysis.",
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