Cerebroside Langmuir monolayers originated from the echinoderms: I. Binary systems of cerebrosides and phospholipids

Hiromichi Nakahara, Shohei Nakamura, Kazufumi Nakamura, Masanori Inagaki, Mariko Aso, Ryuichi Higuchi, Osamu Shibata

研究成果: ジャーナルへの寄稿学術誌査読

23 被引用数 (Scopus)

抄録

The surface pressure (π)-area (A), the surface potential (ΔV)-A and the dipole moment (μ)-A isotherms were obtained for two-component monolayers of two different cerebrosides (LMC-1 and LMC-2) with phospholipids of dipalmitoylphosphatidylcholine (DPPC) and with dipalmitoylphosphatidylethanolamine (DPPE) on a subphase of 0.5 M sodium chloride solution as a function of phospholipid compositions by employing the Langmuir method, the ionizing electrode method, and the fluorescence microscopy. Surface potentials (ΔV) of pure components were analyzed using the three-layer model proposed by Demchak and Fort [J. Colloid Interf. Sci. 46 (1974) 191-202]. The contributions of the hydrophilic saccharide group and the head group to the vertical component of the dipole moment (μ ) were estimated. The miscibility of cerebroside and phospholipid in the two-component monolayers was examined by plotting the variation of the molecular area and the surface potential as a function of the phospholipid molar fraction (Xphospholipid), using the additivity rule. From the A-Xphospholipid and ΔVm-X phospholipid plots, partial molecular surface area (PMA) and apparent partial molecular surface potential (APSP) were determined at the discrete surface pressure. The PMA and APSP with the mole fraction were extensively discussed for the miscible system. Judging from the two-dimensional phase diagrams, these can be classified into two types. The first is a positive azeotropic type; the combinations of cerebrosides with DPPC are miscible with each other. The second is a completely immiscible type: the combination of cerebrosides with DPPE. Furthermore, a regular surface mixture, for which the Joos equation was used for the analysis of the collapse pressure of two-component monolayers, allowed calculation of the interaction parameter (ξ) and the interaction energy (-Δε) between the cerebrosides and DPPC component. The miscibility of cerebroside and phospholipid components in the monolayer state was also supported by fluorescence microscopy.

本文言語英語
ページ(範囲)157-174
ページ数18
ジャーナルColloids and Surfaces B: Biointerfaces
42
2
DOI
出版ステータス出版済み - 5月 10 2005

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学

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