Changes in d-aspartic acid and d-glutamic acid levels in the tissues and physiological fluids of mice with various d-aspartate oxidase activities

Hai Han, Yurika Miyoshi, Reiko Koga, Masashi Mita, Ryuichi Konno, Kenji Hamase

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

d-Aspartic acid (d-Asp) and d-glutamic acid (d-Glu) are currently paid attention as modulators of neuronal transmission and hormonal secretion. These two d-amino acids are metabolized only by d-aspartate oxidase (DDO) in mammals. Therefore, in order to design and develop new drugs controlling the d-Asp and d-Glu amounts via regulation of the DDO activities, changes in these acidic d-amino acid amounts in various tissues are expected to be clarified in model animals having various DDO activities. In the present study, the amounts of Asp and Glu enantiomers in 6 brain tissues, 11 peripheral tissues and 2 physiological fluids of DDO+/+, DDO+/- and DDO-/- mice were determined using a sensitive and selective two-dimensional HPLC system. As a result, the amounts of d-Asp were drastically increased with the decrease in the DDO activity in all the tested tissues and physiological fluids. On the other hand, the amounts of d-Glu were almost the same among the 3 strains of mice. The present results are useful for designing new drug candidates, such as DDO inhibitors, and further studies are expected.

元の言語英語
ページ(範囲)47-52
ページ数6
ジャーナルJournal of Pharmaceutical and Biomedical Analysis
116
DOI
出版物ステータス出版済み - 12 10 2015

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Aspartic Acid
Glutamic Acid
Oxidoreductases
Tissue
Fluids
Acidic Amino Acids
Amino Acids
Mammals
Enantiomers
Viperidae
Pharmaceutical Preparations
Modulators
Brain
Animals
Animal Models
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

これを引用

Changes in d-aspartic acid and d-glutamic acid levels in the tissues and physiological fluids of mice with various d-aspartate oxidase activities. / Han, Hai; Miyoshi, Yurika; Koga, Reiko; Mita, Masashi; Konno, Ryuichi; Hamase, Kenji.

:: Journal of Pharmaceutical and Biomedical Analysis, 巻 116, 10.12.2015, p. 47-52.

研究成果: ジャーナルへの寄稿記事

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AU - Miyoshi, Yurika

AU - Koga, Reiko

AU - Mita, Masashi

AU - Konno, Ryuichi

AU - Hamase, Kenji

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