Changes in metabolic proteins in ex vivo rat retina during glutamate-induced neural progenitor cell induction

Kazuhiro Tokuda, Yasuhiro Kuramitsu, Byron Baron, Takao Kitagawa, Nobuko Tokuda, Masaaki Kobayashi, Kazuhiro Kimura, Koh Hei Sonoda, Kazuyuki Nakamura

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

Understanding how energy metabolism and related proteins influence neural progenitor cells in adult tissues is critical for developing new strategies in clinical tissue regeneration therapy. We have recently reported that a subtoxic concentration of glutamate-induced neural progenitor cells in the mature ex vivo rat retina. We herein explore changes in the metabolic pathways during the process. We firstly observed an increase in lactate and lactate dehydrogenase concentration in the glutamate-treated retina. We then investigated the levels of glycolytic enzymes and confirmed significant upregulation of pyruvate kinase M type (PKM), especially PKM2, enolase, phosphoglycerate mutase 1 (PGAM1), and inosine-5′-monophosphate dehydrogenase (IMPDH1) in the glutamate-treated retina compared to the untreated retina. An analysis of the subcellular localization of PKM2 revealed nuclear translocation in the treated retina, which has been reported to regulate cell cycle proliferation and glycolytic enzymes. Our findings indicate that the mature rat retina undergoes an increase in aerobic glycolysis. PKM2, both in the cytoplasm and in the nucleus, may thus play an important role during neural progenitor cell induction, as it does in other proliferating cells.

本文言語英語
ページ(範囲)177-184
ページ数8
ジャーナルMolecular and cellular biochemistry
419
1-2
DOI
出版ステータス出版済み - 8月 1 2016

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 臨床生化学
  • 細胞生物学

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