Changes in transcription during recovery from heat injury in Salmonella Typhimurium and effects of BCAA on recovery

Hsu Ming Wen, Kimitaka Naito, Yoshimasa Kinoshita, Hiroshi Kobayashi, Ken-ichi Honjoh, Kousuke Tashiro, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Mechanisms of recovery from heat injury in Salmonella typhimurium were elucidated. Recovery of the heat-injured S. typhimurium cells in TSB resulted in full recovery after 3 h of incubation at 37°C. The DNA microarray analysis of 30- and 60-min recovering cells resulted in an increase in transcription of 89 and 141 genes, respectively. Among them, 15 genes, with known function, seemed to be somewhat involved in recovery. They encoded proteins involved in branched-chain amino acid (BCAA) transport (livJ, livH), cell envelope integrity (ddg), heat-shock response (cpxP, rrmJ), phage shock protein (pspA), ribosome modulation factor (rmf), virulence (sseB) transcriptional regulation (rpoE, rpoH, rseA, rseB, rseC) and ArcB signal transduction (sixA) and cytoplasmic membrane protein (fxsA). Among them, the effects of BCAA supplementation on recovery from heat injury were studied to confirm the importance of the BCAA transport liv genes during recovery. It was found that supplementation of TSB with 0.1% BCAA resulted in an enhanced recovery of injured cells in comparison to those recovered in TSB without BCAA. Supplementation of BCAA at 0.1% resulted in a cell count increase 4.4-fold greater than that of the control after 1 h incubation. It seems that BCAA promoted the recovery by promoting protein synthesis either directly through their use in translation or indirectly through stimulation of protein synthesis by activation of the Lrp protein.

元の言語英語
ページ(範囲)2059-2066
ページ数8
ジャーナルAmino Acids
42
発行部数6
DOI
出版物ステータス出版済み - 6 1 2012

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Branched Chain Amino Acids
Salmonella
Salmonella typhimurium
Transcription
Hot Temperature
Recovery
Wounds and Injuries
Genes
Proteins
Enhanced recovery
Heat-Shock Response
Signal transduction
Bacteriophages
Virulence Factors
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
Ribosomes
Shock
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Changes in transcription during recovery from heat injury in Salmonella Typhimurium and effects of BCAA on recovery. / Wen, Hsu Ming; Naito, Kimitaka; Kinoshita, Yoshimasa; Kobayashi, Hiroshi; Honjoh, Ken-ichi; Tashiro, Kousuke; Miyamoto, Takahisa.

:: Amino Acids, 巻 42, 番号 6, 01.06.2012, p. 2059-2066.

研究成果: ジャーナルへの寄稿記事

Wen, Hsu Ming ; Naito, Kimitaka ; Kinoshita, Yoshimasa ; Kobayashi, Hiroshi ; Honjoh, Ken-ichi ; Tashiro, Kousuke ; Miyamoto, Takahisa. / Changes in transcription during recovery from heat injury in Salmonella Typhimurium and effects of BCAA on recovery. :: Amino Acids. 2012 ; 巻 42, 番号 6. pp. 2059-2066.
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abstract = "Mechanisms of recovery from heat injury in Salmonella typhimurium were elucidated. Recovery of the heat-injured S. typhimurium cells in TSB resulted in full recovery after 3 h of incubation at 37°C. The DNA microarray analysis of 30- and 60-min recovering cells resulted in an increase in transcription of 89 and 141 genes, respectively. Among them, 15 genes, with known function, seemed to be somewhat involved in recovery. They encoded proteins involved in branched-chain amino acid (BCAA) transport (livJ, livH), cell envelope integrity (ddg), heat-shock response (cpxP, rrmJ), phage shock protein (pspA), ribosome modulation factor (rmf), virulence (sseB) transcriptional regulation (rpoE, rpoH, rseA, rseB, rseC) and ArcB signal transduction (sixA) and cytoplasmic membrane protein (fxsA). Among them, the effects of BCAA supplementation on recovery from heat injury were studied to confirm the importance of the BCAA transport liv genes during recovery. It was found that supplementation of TSB with 0.1{\%} BCAA resulted in an enhanced recovery of injured cells in comparison to those recovered in TSB without BCAA. Supplementation of BCAA at 0.1{\%} resulted in a cell count increase 4.4-fold greater than that of the control after 1 h incubation. It seems that BCAA promoted the recovery by promoting protein synthesis either directly through their use in translation or indirectly through stimulation of protein synthesis by activation of the Lrp protein.",
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