Channel wall coating on a poly-(methyl methacrylate) CE microchip by thermal immobilization of a cellulose derivative for size-based protein separation

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

We demonstrate channel wall coating using a cellulose derivative on a poly-(methyl methacrylate) (PMMA) CE microchip to eliminate EOF disturbing protein separation. The channel walls were modified by preconditioning with a solution containing the cellulose derivative and then thermally evaporating the solution to produce hydrophilic channel walls which prevent adsorption of analytes via a hydrophobic interaction. When the PMMA substrate was coated with the cellulose derivative hydroxypropylmethylcellulose (HPMC) 90SH, the water contact angle on the coated substrate was decreased (up to 15°) and EOF was significantly suppressed (up to 4.0 × 10-6 cm2·V-1·s-1). Three proteins (20.5, 68.0, and 114.6 kDa) were successfidly separated on the 0.15% HPMC 90SH-coated channel walls with good reproducibility of migration time (RSD <1.75%) and high efficiency (theoretical plate number per meter: 2.62 × 105).

本文言語英語
ページ(範囲)4582-4589
ページ数8
ジャーナルELECTROPHORESIS
28
24
DOI
出版ステータス出版済み - 12月 2007
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 臨床生化学

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