Channels formed by amphotericin B covalent dimers exhibit rectification

Minako Hirano, Yuko Takeuchi, Nobuaki Matsumori, Michio Murata, Toru Ide

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Amphotericin B (AmB) is a widely used antifungal antibiotic with high specificity for fungi. We previously synthesized several covalently conjugated AmB dimers to clarify the AmB channel structure. Among these dimers, that with an aminoalkyl linker was found to exhibit potent hemolytic activity. We continue this work by investigating the channel activity of the dimer, finding that all channels comprised of AmB dimers show rectification. The direction of the dimer channel in the membrane depended on the electric potential at which the dimer channel was formed. On the other hand, only about half the monomer channels showed rectification. In addition, these channels were easily switched from a rectified to a nonrectified state following voltage stimulation, indicating instability. We propose a model to describe the AmB channel structure that explains why AmB dimer channels necessarily show rectification.

本文言語英語
ページ(範囲)159-164
ページ数6
ジャーナルJournal of Membrane Biology
240
3
DOI
出版ステータス出版済み - 4 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生理学
  • 細胞生物学

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