Characteristics of new bacillus thuringiensis strains isolated From soils in the vicinity of the Baekdu mountain and biological activity against Spodoptera exigua

Yu Seop Kim, Na Young Jin, You Kyoung Lee, Hee Ji Kim, Young A. Hur, Young Nam Youn, Chisa Yasunaga-Aoki, Dae Yong Kim, And Yong Man Yu

研究成果: ジャーナルへの寄稿記事

抄録

From among 16 B. thuringiensis strains collected and isolated from soils in the vicinity of the Baekdusan mountain, four strains (YN1-1, YN2-6, YN4-2, and YN6-2) that showed high LC95 values for Spodoptera exigua. 3rd larvae of 2.43 × 106, 7.00 × 106, 3.26 × 106, and 2.62 × 106 cfu/ml, respectively, were finally selected. SDS-PAGE experiments were conducted to identify the toxoprotein patterns of the new four strains and, in the results, all the strains showed major bands of ca. 130 kDa, which were changed into ca. 65 kDa through digestion by Spodoptera exigua midgut fluid. To identify the Cry gene compositions of the strains, plasmid DNAs were extracted and PCR was conducted using specific primers (crylAa-crylL). Six genes (crylAa, crylAb, crylC, crylD, cryll and crylL) were identified in three strains: YN1-1, 2-6, and 4-2 and the amplification of five genes; crylAa, crylAc, crylC, crylD and crylL in the YN6-2 strain. All four B. thuringiensis strains were identified as B. thuringiensis subsp. aizawai strains by comparing the H serotypes of 16S rRNA and flagellin (fliC) gene. The possibility of commercialization of YN1-1 strain was examined by insecticidal activity test for the Spodoptera exigua 3rd larvae of six B. thuringiensis pesticide products sold in Korea.

元の言語英語
ページ(範囲)419-428
ページ数10
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
62
発行部数2
出版物ステータス出版済み - 9 1 2017

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Spodoptera
Bacillus thuringiensis
Spodoptera exigua
bioactive properties
Soil
mountains
Larva
soil
Genes
Flagellin
Gene Amplification
Korea
Pesticides
Polyacrylamide Gel Electrophoresis
Digestion
Plasmids
Bacillus thuringiensis subsp. aizawai
Polymerase Chain Reaction
DNA
gene amplification

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Characteristics of new bacillus thuringiensis strains isolated From soils in the vicinity of the Baekdu mountain and biological activity against Spodoptera exigua. / Kim, Yu Seop; Jin, Na Young; Lee, You Kyoung; Kim, Hee Ji; Hur, Young A.; Youn, Young Nam; Yasunaga-Aoki, Chisa; Kim, Dae Yong; Yu, And Yong Man.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 62, 番号 2, 01.09.2017, p. 419-428.

研究成果: ジャーナルへの寄稿記事

Kim, Yu Seop ; Jin, Na Young ; Lee, You Kyoung ; Kim, Hee Ji ; Hur, Young A. ; Youn, Young Nam ; Yasunaga-Aoki, Chisa ; Kim, Dae Yong ; Yu, And Yong Man. / Characteristics of new bacillus thuringiensis strains isolated From soils in the vicinity of the Baekdu mountain and biological activity against Spodoptera exigua. :: Journal of the Faculty of Agriculture, Kyushu University. 2017 ; 巻 62, 番号 2. pp. 419-428.
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abstract = "From among 16 B. thuringiensis strains collected and isolated from soils in the vicinity of the Baekdusan mountain, four strains (YN1-1, YN2-6, YN4-2, and YN6-2) that showed high LC95 values for Spodoptera exigua. 3rd larvae of 2.43 × 106, 7.00 × 106, 3.26 × 106, and 2.62 × 106 cfu/ml, respectively, were finally selected. SDS-PAGE experiments were conducted to identify the toxoprotein patterns of the new four strains and, in the results, all the strains showed major bands of ca. 130 kDa, which were changed into ca. 65 kDa through digestion by Spodoptera exigua midgut fluid. To identify the Cry gene compositions of the strains, plasmid DNAs were extracted and PCR was conducted using specific primers (crylAa-crylL). Six genes (crylAa, crylAb, crylC, crylD, cryll and crylL) were identified in three strains: YN1-1, 2-6, and 4-2 and the amplification of five genes; crylAa, crylAc, crylC, crylD and crylL in the YN6-2 strain. All four B. thuringiensis strains were identified as B. thuringiensis subsp. aizawai strains by comparing the H serotypes of 16S rRNA and flagellin (fliC) gene. The possibility of commercialization of YN1-1 strain was examined by insecticidal activity test for the Spodoptera exigua 3rd larvae of six B. thuringiensis pesticide products sold in Korea.",
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AU - Jin, Na Young

AU - Lee, You Kyoung

AU - Kim, Hee Ji

AU - Hur, Young A.

AU - Youn, Young Nam

AU - Yasunaga-Aoki, Chisa

AU - Kim, Dae Yong

AU - Yu, And Yong Man

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